Looking on the datasheet, it states that there is 250 ug of DNA per vial and that the vial has 100 x 5uL loads (500 uL volume). DNA concentration is 0.5 ug/uL. Loading 5 uL per well therefore provides a DNA load of 2.5 ug per well. Please view the data sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/157/148/d3937dat.pdf
D3937
DirectLoad™ 1 kb DNA Ladder
ready-to-use marker for DNA electrophoresis
Sinonimo/i:
1 kb marker, 1kb gel marker, DNA marker, agarose gel electrophoresis marker
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 1 vial | Per conoscere la disponibilità, visualizza il carrello | CHF 268.00 |
Informazioni su questo articolo
NACRES:
NA.25
UNSPSC Code:
41105335
CHF 268.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartiQuality Segment
form
liquid
usage
100 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.
Application
Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
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Questo articolo | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| usage 100 uses | usage 75 uses | usage 100 uses | usage sufficient for 50 uses |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
NA electrophoresis | - | NA electrophoresis, ladder, marker | - |
Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocolli
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Articoli
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Contenuto correlato
Datasheet
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| D3937-1VL | 04061835572588 |
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