C8868
Cholesterol Oxidase from Brevibacterium
lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli
Sinonimo/i:
Cholesterol: oxygen oxidoreductase
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 100 units | Per conoscere la disponibilità, visualizza il carrello | CHF 202.00 | |
| 500 units | Per conoscere la disponibilità, visualizza il carrello | CHF 683.00 |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-842-1
MDL number:
Specific activity:
≥50 units/mg protein
Recombinant:
expressed in E. coli
CHF 202.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartirecombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
≥50 units/mg protein
mol wt
64 kDa
composition
Protein, ≥15% biuret
storage temp.
−20°C
General description
Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme.
Application
Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.
Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]
Biochem/physiol Actions
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.[7]
Physical form
Supplied as a lyophilzed powder containing sucrose
Other Notes
One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.
1 of 1
Questo articolo | |||
|---|---|---|---|
| specific activity ≥50 units/mg protein | specific activity ≥10 units/mg protein | specific activity ≥30 units/mg protein (biuret) | specific activity 8-20 units/mg solid |
| form lyophilized powder | form lyophilized powder | form aqueous solution | form lyophilized powder |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| mol wt 64 kDa | mol wt 55 kDa | mol wt 62 kDa | mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocolli
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
Contenuto correlato
Instructions
Block copolymer microdomains: a novel medium for enzymatic reactions
Gupte, A., et al.
Biotechnology Progress, 7, 348-354 (2012)
Meihe Zhang et al.
Biosensors & bioelectronics, 32(1), 288-292 (2011-12-31)
A novel cholesterol biosensor was prepared based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence (ECL). Firstly, l-cysteine-reduced graphene oxide composites were modified on the surface of a glassy carbon electrode. Then, gold nanoparticles (AuNPs) were self-assembled on it. Subsequently, cholesterol oxidase
Laura Caldinelli et al.
The Journal of biological chemistry, 280(24), 22572-22581 (2005-04-09)
Cholesterol oxidase from Brevibacterium sterolicum is a monomeric flavoenzyme catalyzing the oxidation and isomerization of cholesterol to cholest-4-en-3-one. This protein is a class II cholesterol oxidases, with the FAD cofactor covalently linked to the enzyme through the His(69) residue. In
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| C8868-500UN | 04061833522950 |
| C8868-100UN | 04061833522943 |