C8176
Collagenasi
Sigma Blend Type L, ≤1.0 FALGPA units/mg solid
Sinonimo/i:
Clostridiopeptidasi A
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About This Item
Prodotti consigliati
Livello qualitativo
Forma fisica
powder
caseinase activity
≥40 units/mg solid
Attività specifica
≤1.0 FALGPA units/mg solid
PM
68-130 kDa
Temperatura di conservazione
−20°C
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Applicazioni
Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess the synthesis and in vitro evaluation of macromolecular antitumour derivatives based on phenylenediamine mustard. Clostridiopeptidase A has also been used in a study to investigate the enzymatic debridement of burn wounds with collagenase.
Azioni biochim/fisiol
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.
La collagenasi è attivata da quattro grammi atomo di calcio per mole di enzima. È inibita da etilen-glicol-bis(beta-amminoetil etere) - acido N, N, N′,N′-tetraacetico, beta-mercaptoetanolo, glutatione, acido tioglicolico e 8-idrossichinolina.
Definizione di unità
Una unità di digestione del collagene (CDU) libera peptidi da collagene proveniente da tendine d′Achille bovino equivalente in colore ninidrina a 1,0 μmole di leucina in 5 ore a pH 7,4 a 37 °C in presenza di ioni calcio. Una unità di idrolisi FALGPA idrolizza 1,0 μmole di furilacriloil-Leu-Gly-Pro-Ala per minuto a 25 °C. Una unità di proteasi neutra idrolizza caseina e produce colore equivalente a 1,0 μmole di tirosina per 5 ore a pH 7,5 a 37 °C. Una unità di clostripaina idrolizza 1,0 μmole of BAEE per minuto a pH 7,6 a 25 °C in presenza di DTT.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificati d'analisi (COA)
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I clienti hanno visto anche
Coşkun Ozcan et al.
Burns : journal of the International Society for Burn Injuries, 28(8), 791-794 (2002-12-05)
Seventy-eight pediatric burn patients treated by enzymatic debridement with collagenase clostridiopeptidase A (CCA), were compared to 41 patients those burn wounds were excised surgically. Patients whose burn wounds were initially assessed as partial-thickness at admission were enrolled in the study.
N I Solov'eva et al.
Bioorganicheskaia khimiia, 20(3), 303-309 (1994-03-01)
Interactions of collagenases I and II (clostridiopeptidases) from Clostridium histolyticum with hexapeptide substrates in which some L-proline residues are replaced by their D-analogues, as well as with the tripeptide chloromethyl ketone Z-Gly-Pro-Gly-CH2Cl were studied. A role of stereochemistry of the
Ruth M Williams et al.
STAR protocols, 2(2), 100414-100414 (2021-04-20)
In order to process samples by fluorescence-activated cell sorting (FACS), it is essential to obtain a single-cell suspension of dissociated cells. Numerous protocols and commercial reagents are available; however, each requires optimization for specific tissue types. Here, we describe an
Katleen De Winne et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 24(2-3), 159-168 (2005-01-22)
Poly-[N-(2-hydroxyethyl)-L-glutamine] (PHEG) and poly(ethylene glycol) (PEG)-grafted PHEG conjugates of N,N-di(2-chloroethyl)-4-phenylenediamine mustard (PDM) were synthetised. A collagenase-sensitive oligopeptide spacer was selected to link the cytotoxic agent PDM onto the polymeric carrier. First, the oligopeptide-drug conjugate, L-pro-L-leu-gly-L-pro-gly-PDM, was prepared. In a second
Dag K Skovseth et al.
Methods in molecular biology (Clifton, N.J.), 360, 253-268 (2006-12-19)
The future ability to manipulate the growth of new blood vessels (angiogenesis) holds great promise for treating ischemic disease and cancer. Several models of human in vivo angiogenesis have been described, but they seem to depend on transgenic support and
Protocolli
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
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