This product is quite stable and is not assigned an expiration or retest date. A one-year warranty from the date of shipment applies. Provided that the product is handled appropriately, securely sealed, and not contaminated, the material is stable for at least the 1 year warranty period, both opened and unopened. Please see the link below to review the full Product Dating Information Statement:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/356/113/product-dating-information-06-25-ms.pdf
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
100 g | Per conoscere la disponibilità, visualizza il carrello | CHF 71.10 |
500 g | Per conoscere la disponibilità, visualizza il carrello | CHF 261.00 |
Informazioni su questo articolo
Quality Segment
form
emulsion (aqueous)
concentration
10% (active silicon)
technique(s)
cell culture | hybridoma: suitable
solubility
H2O: soluble (effective hot or cold.)
density
1.0 g/mL at 25 °C
application(s)
microbiology
storage temp.
room temp
suitability
(Mammalian (suspension)), bacteria (fermentation)
General description
Application
- nella preparazione del tampone di amplificazione per la tecnica di replicazione a cerchio rotante (RAM) ramificato;
- per valutarne gli effetti su vitalità cellulare, infettività virale e prestazioni della reazione a catena della polimerasi (PCR);
- come componente del tampone fosfato salino (PBS) per determinare lo stato antiossidante totale (TAOS) mediante il saggio con acido 2,2′-azino-bis(3-etilbenzotiazolin-6-solfonico (ABTS); [1]
- come componente del terreno di autoinduzione dell'espressione delle colture;[2]
- come componente del tampone di rilevazione dell'adenosina monofosfato ciclico (cAMP). [3]
Features and Benefits
- Controlla la formazione di schiuma in fiasche per colture o fermentazioni microbiche
- Non tossico per il terreno di coltura
- Può essere pompato nel fermentatore al bisogno
Preparation Note
devono essere utilizzate immediatamente.
Other Notes
1 of 1
Questo articolo | |||
|---|---|---|---|
| suitability (Mammalian (suspension)), bacteria (fermentation) | suitability (Mammalian (suspension)), bacteria (fermentation) | suitability (Mammalian (suspension)), bacteria (fermentation) | suitability - |
| technique(s) cell culture | hybridoma: suitable | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable | technique(s) - | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable |
| form emulsion (aqueous) | form emulsion (aqueous) | form emulsion (aqueous) | form emulsion |
| application(s) microbiology | application(s) microbiology | application(s) microbiology | application(s) - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
| solubility H2O: soluble (effective hot or cold.) | solubility - | solubility - | solubility - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - STOT RE 2 Inhalation
target_organs
Lungs
Classe di stoccaggio
12 - Non Combustible Liquids
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What is the shelf-life of product A8582 unopened as well as after the bottle is opened and first used? Is there a dependency of that shelf-life on the date of manufacture? And where can the customer access that information?
1 answer-
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細胞培養液に添加するためにアンチフォームSE-15の購入を検討しております。この商品は、オートクレーブ可能でしょうか、オートクレーブ可能の場合何回程度が限度でしょうか。 もし可能であれば、アンチフォーム C エマルジョンとの違いも教えていただけないでしょうか。 (アンチフォーム C エマルジョンもオートクレーブ可能でしょうか。) よろしくお願い申し上げます。
1 answer-
Antifoam SE-15 is a 10% emulsion of active silicone polymer and non-ionic emulsifiers. It can be repeatedly sterilized by autoclaving. Antifoam C Emulsion is an aqueous emulsion containing 30% active silicone. Further details about the different properties can be found in the datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/313/a8582pis.pdfHelpful?
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At what concentration is SE-15 (A8582) to be used in bacterial cell culture to control foam?
1 answer-
This Antifoam product is typically used at starting concentrations of 0.01 - 0.02%. Optimization may be required based on variable culture density, ingredients, and conditions. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/133/175/a8582dat.pdfHelpful?
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I was wondering if you had any information in regards to the stability of antifoam SE-15, A8582. In particular, do you have any data about the stability of this product at pH 8.0? When in a solution, do you have any data on the product stability?
1 answer-
This product is stable at room temperature. Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted suspensions should be used immediately. The stability of this product is not tested at pH 8.0. If you are looking for antifoams that will be stable at pH 8.0, perhaps you may consider Antifoam A Concentrate (Product A5633 and A6582), They are stable at pH 5.0-9.0. Please see this link for any additional information on Product A8582: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/313/a8582pis.pdf
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