A3150
Fosfatasi alcalina anti-IgG umane (specifica per la catena γ)−
affinity isolated antibody, buffered aqueous glycerol solution
Sinonimo/i:
Goat Anti-Human IgG (γ-chain specific)−AP
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About This Item
Prodotti consigliati
Origine biologica
goat
Livello qualitativo
Coniugato
alkaline phosphatase conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous glycerol solution
Reattività contro le specie
human
tecniche
direct ELISA: 1:7,000-1:21,000
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
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Descrizione generale
Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.
Immunogeno
purified human IgG
Applicazioni
Le proteine raccolte da colture di E. coli sono state analizzate su gel SDS-page nel western blotting utilizzando IgG di capra anti-umana (specifica per la catena) coniugata con fosfatasi alcalina a una concentrazione di 1:10 000 per il secondario. L′IgG è stata rilevata nel siero umano con Elisa utilizzando IgG di capra anti-umana (specifica per la catena gamma) coniugata con fosfatasi alcalina diluita 1:5000 in 0,25% BSA/PBS. Il saggio è stato sviluppato con p-nitrofenil fosfato come substrato (1 mg/mL Sigma).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody can be used for ELISA applications using serum samples from allergic and borderline hypertensive patients.
Azioni biochim/fisiol
Human IgGs regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones Kappa, and Lambda myeloma proteins.
IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions. IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region aids in the development of therapeutic antibody.
Stato fisico
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
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