A3525
Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
Sinonimo/i:
Goat Anti-Human IgE (ε-chain specific)−AP
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0.5 ML
CHF 311.00
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CHF 532.00
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0.5 ML
CHF 311.00
1 ML
CHF 532.00
About This Item
Prodotti consigliati
Origine biologica
goat
Coniugato
alkaline phosphatase conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Stato
buffered aqueous glycerol solution
Reattività contro le specie
human
tecniche
direct ELISA: 1:7,000-1:21,000
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Affinity isolated antibody is obtained from anti-human IgE antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the ε-chain of human IgE. Anti-Human IgE is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. IgE is present in low levels compared to other immunoglobulin isotypes. It exists in two forms, one being a secreted by plasma cell and the other form is associated with the B cell receptor.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma . Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is specific for human IgE when tested against human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins.
Immunogeno
Purified human IgE
Applicazioni
Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat has been used in dot blot.
Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is suitable for use in immunoblotting and ELISA (at 1:500 dilutions using 100μ/well).
IgE secreted from transfected murin myeloma cells was detected by ELISA using the alkaline phosphatase-conjugated goat anti-human IgE (epsilon specific) antibody.
Azioni biochim/fisiol
IgE bound to B cell receptor mediates antigen uptake and regulates differentiation and proliferation of B cells.
Stato fisico
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Prodotti correlati
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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