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Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Biological source:
potato
biological source
potato
form
lyophilized powder
quality
High Activity
ATPase activity
≥600 units/mg protein
secondary activity
≥50 % of base activity ADPase
composition
protein, ≥30%
foreign activity
Acid Phosphatase ≤2% of base activity
shipped in
wet ice
storage temp.
−20°C
Application
In diverse varietà di S. tuberosum si trovano almeno due isoenzimi: uno con un rapporto ATPasi/ADPasi elevato (∼10) e un altro con un rapporto basso (∼1).
Reazione: ATP → ADP+Pi → AMP+2Pi.
Reazione: ATP → ADP+Pi → AMP+2Pi.
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1]. Product A2230 is a high activity apyrase.
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Packaging
Sold on the basis of ATPase units.
Physical form
Lyophilized powder containing potassium succinate buffer salts.
Preparation Note
Derived from red potato
Other Notes
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
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Questo articolo | |||
|---|---|---|---|
| biological source potato | biological source potato | biological source - | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| foreign activity Acid Phosphatase ≤2% of base activity | foreign activity - | foreign activity - | foreign activity Acid Phosphatase ≤2% of base activity |
| composition protein, ≥30% | composition Protein, 30-60% | composition - | composition Protein, 25-60% |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenuto correlato
Instructions
David W Rooklin et al.
Journal of the American Chemical Society, 134(37), 15595-15603 (2012-08-30)
Human soluble calcium-activated nucleotidase 1 (hSCAN-1) represents a new family of apyrase enzymes that catalyze the hydrolysis of nucleotide di- and triphosphates, thereby modulating extracellular purinergic and pyrimidinergic signaling. Among well-characterized phosphoryl transfer enzymes, hSCAN-1 is unique not only in
Jing Wu et al.
Investigative ophthalmology & visual science, 53(11), 7142-7148 (2012-09-22)
To investigate the mechanisms for endogenous production of extracellular adenosine in trabecular meshwork (TM) cells and evaluate its physiological relevance to the regulation of aqueous humor outflow facility. Extra-cellular levels of adenosine monophosphate (AMP) and adenosine in porcine trabecular meshwork
Thomas J Bushart et al.
American journal of botany, 100(1), 161-174 (2012-10-11)
Gravity regulates the magnitude and direction of a trans-cell calcium current in germinating spores of Ceratopteris richardii. Blocking this current with nifedipine blocks the spore's downward polarity alignment, a polarization that is fixed by gravity ∼10 h after light induces
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| A2230-100UN | 04061833349861 |




