A1009
Albumin methylated from bovine serum
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About This Item
Prodotti consigliati
Origine biologica
bovine
Livello qualitativo
Forma fisica
powder
Origine
USA origin
tecniche
affinity chromatography: suitable
fractionation: suitable
Impurezze
≤10% water (Karl Fischer)
N° accesso UniProt
Temperatura di conservazione
2-8°C
Informazioni sul gene
bovine ... ALB(280717)
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Applicazioni
Albumin methylated from bovine serum (mBSA) has been used as a coating for immunoplates in enzyme-linked immunosorbent assay (ELISA) of anti-dsDNA IgG antibodies. It has also been used to induce arthritis in mice models.
As an affinity ligand on diatomaceous earth support (MAK chromatography), a medium for fractionation of different forms of DNA and for separating aminoacyl-tRNAs. Different modes of binding of double-stranded, denatured, and replicating forms of DNA account for the efficacy of the method.
Azioni biochim/fisiol
Albumin is a major circulating component of the plasma and is a highly soluble multifunctional protein. It plays a role in several physiological and pharmacological processes. Albumin binds to metals, cholesterol, hormones, bile pigments, drugs, and fatty acids to facilitate their transport. It regulates osmotic pressure and distributes fluids between various compartments.
Nota sulla preparazione
Methyl ester of albumin prepared per Mandell, J.D. and Hershey, A.D., Anal. Biochem., 1, 66 (1960).
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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Kasper Almholt et al.
Journal of immunology (Baltimore, Md. : 1950), 200(3), 957-965 (2017-12-29)
Genetic absence of the urokinase-type plasminogen activator (uPA) reduces arthritis progression in the collagen-induced arthritis (CIA) mouse model to an extent just shy of disease abrogation, but this remarkable observation has not been translated into therapeutic intervention. Our aim was
T Hofstaetter et al.
Biochimica et biophysica acta, 518(1), 53-60 (1978-03-29)
The binding of methylated albumin to DNA, the basis of the chromatography on columns of kieselguhr coated with methylated albumin (MAK chromatography), was investigated. Scatchard plots revealed only one mode of interaction with fully double-stranded DNA. The complexes should be
Fractionation of nucleic acids with the methylated albumin column.
N SUEOKA et al.
Journal of molecular biology, 4, 161-172 (1962-03-01)
David L Mitchell
Methods in molecular biology (Clifton, N.J.), 314, 239-249 (2006-05-06)
Over the past 20 yr, the use of polyclonal and monoclonal antibodies to quantify damage in DNA has burgeoned. Immunoassays offer distinct advantages over other anaytical procedures currently used to measure DNA damage including adaptability, sensitivity and selectivity. This combination
Mohammad Haque et al.
Scientific reports, 6, 20588-20588 (2016-02-06)
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