62309

Lipase from Pseudomonas cepacia

powder, light beige, ≥30 U/mg

• Sinonimo/i: PCL, PS Lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numero CAS: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-619-9
• Numero CE: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509
• Specific activity: ≥30 U/mg
• Biological source: bacterial (Pseudomonas cepacia)

Proprietà

• biological source: bacterial (Pseudomonas cepacia)
• Quality Segment: 100
• form: powder
• specific activity: ≥30 U/mg
• storage condition: (Tightly closed. Dry)
• technique(s): cell based assay: suitable
• color: light beige
• solubility: H₂O: 2 mg/mL, hazy, faintly yellow
• UniProt accession no.: A0A0J5WKE3
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Descrizione

General description

Research area: cell-signaling

Lipase is a hydrolytic enzyme, found ubiquitously in nature. It belongs to the α/β-hydrolases fold family.[1] Lipase structure contains amphipathic helical lid domain in the active site that helps in interfacial activation of protein.[2]

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Pseudomonas cepacian has been used to:

• catalyze the degradation of polycaprolactone scaffold[3]
• catalyze the hydrolysis of Morita-Baylis-Hillman acetates during enzymatic kinetic resolution of racemic Morita-Baylis-Hillman adducts
• as a standard for the generation of a calibration curve to determine the activity of lipase produced by microorganisms isolated from sludge derived from an urban wastewater treatment plant for ethanol production.[4]

Biochem/physiol Actions

Lipases catalyze the hydrolysis of carboxylic ester bonds in triacylglycerols to yield glycerol and free fatty acids.[5] Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Triacylglycerol lipases specifically hydrolyze the outer links of triacylglycerols and operate exclusively on the water-lipid interface. Lipolytic products and intermediates formed during lipolysis are involved in various cell-signaling processes.[6] Lipases have broad substrate specificity and high enantioselectivity.[7] This property of lipase makes it a good catalyst in organic synthesis.[1] Lipases play a vital role in fat digestion and metabolism.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)

Chemoenzymatic synthesis of (-)-carbocyclic 7-deazaoxetanocin G.

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Informazioni sulla sicurezza

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)