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37291

Dihydroethidium

BioReagent, suitable for fluorescence, ≥95% (HPCE)

Sinonimo/i:

2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

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Cambia visualizzazione
Taglio della confezioneSKUDisponibilitàPrezzo
25 mg
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CHF 319.00
100 mg
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CHF 802.00

Informazioni su questo articolo

Formula empirica (notazione di Hill):
C21H21N3
Numero CAS:
Peso molecolare:
315.41
UNSPSC Code:
12161505
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5107482

CHF 319.00


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product line

BioReagent

Quality Segment

assay

≥95% (HPCE)

form

solid

mol wt

315.41 g/mol

solubility

acetonitrile: soluble, methanol: soluble

fluorescence

λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

Application

Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.
Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

B-ring reduced analog of ethidium

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Questo articolo
D700866807E7637
Dihydroethidium BioReagent, suitable for fluorescence, ≥95% (HPCE)

Sigma-Aldrich

37291

Dihydroethidium

Dihydroethidium ≥95%

Sigma-Aldrich

D7008

Dihydroethidium

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

suitability

-

fluorescence

λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol

fluorescence

λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells)

fluorescence

λex 367 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (after derivatization with pyruvate)

fluorescence

λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;), λex 526 nm; λem 605 nm in aqueous buffer

solubility

acetonitrile: soluble, methanol: soluble

solubility

chloroform: 20 mg/mL

solubility

-

solubility

-

form

solid

form

powder

form

solid

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

assay

≥95% (HPCE)

assay

≥95%

assay

≥97.0% (HPLC)

assay

-


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Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Assessment of low-level superoxide in nonphagocytic cells is crucial for assessing redox-dependent signaling pathways and the role of enzymes such as the NADPH oxidase complex. However, most superoxide probes present inherent limitations. Particularly, assessment of dihydroethidium (DHE) fluorescence is limited



Numero articolo commerciale globale

SKUGTIN
15382-25G04061838741141
15382-5G04061832593715
853057010004027269098254
15382-100G04061832593708
853057002504027269098247
37291-25MG04061831834352
37291-100MG04061833488911

Questions

  1. How should stock solutions for item 37291, Dihydroethidium, be stored?

    1 answer
    1. The material should be soluble in ethanol, with dichloromethane (DCM) being a commonly used solvent for it.

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