07-689

Anti-EZH2 Antibody

Upstate^®, from rabbit

• Sinonimo/i: KMT6, Enx1, Enhancer of Zeste Homologue 2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ChIP, IP, WB
• Citations: 66

Proprietà

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NM_004456.3, NM_152998.1
• UniProt accession no.: Q15910
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... EZH2(2146)

Descrizione

General description

95-100kDa

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2) is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.

Immunogen

His-tagged fusion protein corresponding to residues 1-343 of mouse enhancer of Zeste homolog 2 (EZH2)

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-EZH2 Antibody is validated for use in Immunoprecipitation and Western Blotting and ChIP-seq for the detection of EZH2.

Biochem/physiol Actions

EZH2

Physical form

Antiserum

Format: Purified

Preparation Note

stable for 2 years at 2-8°C from date of shipment

Analysis Note

Control
K562 cell lysate, C2C12 cell lysate

routinely evaluated by immunoblot on RIPA lysates from HeLa and C2C12 cells

Other Notes

feature_concentration_valuePlease refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable