07-353
Anticorpo anti-acetil-istone H3 (Lys14)
serum, Upstate®
Sinonimo/i:
H3K14Ac, Histone H3 (acetyl K14), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
Scegli un formato
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 100 μL | Per conoscere la disponibilità, visualizza il carrello | CHF 547.00 |
Informazioni su questo articolo
CHF 547.00
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
Saccharomyces cerevisiae, yeast, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq), dot blot: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
acetylation (Lys14)
Gene Information
human ... H3C1(8350)
General description
Immunogen
Application
Epigenetica & funzione nucleare
un laboratorio indipendente ha dimostrato che questo anticorpo immunoprecipita preferenzialmente la cromatina di lievito wild-type e non di ceppi di lievito portatori di una sostituzione della lisina con alanina nel residuo 14.
Saggio di specificità istone-peptide Beadlyte:
diluizioni 1:1.000-1:5.000 di un precedente lotto sono state incubate con peptidi istonici H3 contenenti varie modifiche e coniugati a microsfere Luminex: Non è stata osservata reattività crociata con peptidi contenenti acetil-lisina 9 o acetil-lisina 27.
Istoni
Biochem/physiol Actions
Physical form
Preparation Note
Per ottimizzare il recupero del prodotto, centrifugare il flaconcino originale dopo lo scongelamento e prima di togliere il tappo.
Analysis Note
Lisato di cellule C6, lisato di cellule NIH 3T3, carcinoma mammario umano e carcinoma polmonare umano.
Saggio di Western blotting:
questo lotto alla diluizione 1:1.000-1:5.000 ha permesso di rilevare l′acetil-istone H3 (Lys14) in estratti acidi di cellule HeLa trattate con butirrato di sodio (N° Cat. 17-305).
Other Notes
Legal Information
Disclaimer
1 of 1
Questo articolo | |||
|---|---|---|---|
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| species reactivity Saccharomyces cerevisiae, yeast, human | species reactivity mouse, human, rat | species reactivity human, Saccharomyces cerevisiae, yeast, vertebrates | species reactivity mouse, rat, human |
| antibody form serum | antibody form purified immunoglobulin | antibody form serum | antibody form purified antibody |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| Gene Information human ... H3C1(8350) | Gene Information human ... HIST1H3F(8968) | Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) |
| technique(s) ChIP: suitable (ChIP-seq), multiplexing: suitable, dot blot: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), dot blot: suitable, flow cytometry: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), multiplexing: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, western blot: suitable |
Still not finding the right product?
Provate il nostro Motore di ricerca dei prodotti per restringere le opzioni.
Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Contenuto correlato
Histone Modifications Poster
Cancer is a complex disease manifestation. At its core, it remains a disease of abnormal cellular proliferation and inappropriate gene expression. In the early days, carcinogenesis was viewed simply as resulting from a collection of genetic mutations that altered the gene expression of key oncogenic genes or tumor suppressor genes leading to uncontrolled growth and disease (Virani, S et al 2012). Today, however, research is showing that carcinogenesis results from the successive accumulation of heritable genetic and epigenetic changes. Moreover, the success in how we predict, treat and overcome cancer will likely involve not only understanding the consequences of direct genetic changes that can cause cancer, but also how the epigenetic and environmental changes cause cancer (Johnson C et al 2015; Waldmann T et al 2013). Epigenetics is the study of heritable gene expression as it relates to changes in DNA structure that are not tied to changes in DNA sequence but, instead, are tied to how the nucleic acid material is read or processed via the myriad of protein-protein, protein-nucleic acid, and nucleic acid-nucleic acid interactions that ultimately manifest themselves into a specific expression phenotype (Ngai SC et al 2012, Johnson C et al 2015). This review will discuss some of the principal aspects of epigenetic research and how they relate to our current understanding of carcinogenesis. Because epigenetics affects phenotype and changes in epigenetics are thought to be key to environmental adaptability and thus may in fact be reversed or manipulated, understanding the integration of experimental and epidemiologic science surrounding cancer and its many manifestations should lead to more effective cancer prognostics as well as treatments (Virani S et al 2012).
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| 07-353 | 04053252588303 |