T7201

Trombina

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

• Sinonimo/i: Fattore IIa
• Numero CAS: 9002-04-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numero CE: 3.4.21.5 (BRENDA, IUBMB)
• MDL number: MFCD00166053
• Specific activity: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

Proprietà

• form: lyophilized powder
• specific activity: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
• mol wt: heavy chain ~31 kDa, light chain ~6 kDa
• storage temp.: −20°C

Descrizione

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin consists of A-chain and a catalytic B-chain with the catalytic pocket comprising of His57, Asp102, Ser195 and Ser214. It has a fibrinogen recognition site (FRS) and a heparin binding site (HBS) in the C-terminus. The main function of thrombin is cleavage of fibrinogen to fibrin. It coordinates the coagulation cascade and binds to diverse substrates. It coordinates with protease-activated receptors (PARs) to accomplish the physiological functions. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contribute to the progression of Parkinson′s disease. Altered thrombin levels modulates coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess a novel antiplatelet therapy in acute coronary syndromes. [1]

Thrombin from bovine plasma has been used in the cleavage of the maltose binding protein tag in mini-calpain-5 fusion protein and in thrombin/hirudin spiking experiments in serum samples.

Biochem/physiol Actions

Serin proteasi che scinde in modo selettivo i legami Arg-Gly nel fibrinogeno per formare fibrina e i fibrinopeptidi A e B.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Preparation Note

Traditional thrombin products are activated with bovine brain, whereas T7201 is activated with bovine lung and does not contain any bovine brain products.

Analysis Note

La procedura del saggio NIH impiega 0,2 mL di plasma diluito (1:1 con soluzione fisiologica) come substrato e 0,1 mL di campione di trombina (stabilizzato in una soluzione di albumina tamponata all′1%) sulla base di una modificazione del metodo di Biggs. Per determinare le concentrazioni di trombina si usano solo tempi di coagulazione nell′intervallo di 15-25 secondi.

L′attività è espressa in unità NIH ottenute per confronto diretto con uno Standard di riferimento della trombina NIH, Lotto K.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)