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Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Sinonimo/i:

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

Codice UNSPSC:
41105319
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Sterilità

0.2 μm filtered

Forma fisica

solution

Attività estranea

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

Temperatura di conservazione

room temp

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Categorie correlate

Descrizione generale

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Applicazioni

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Caratteristiche e vantaggi

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Componenti

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Protocolli

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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