No, this product does not contain a His-tag. See the link below for additional product information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/394/024/p7582dat.pdf
P7582
Amino-terminal FLAG-BAP™ Fusion Protein
Sinonimo/i:
Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein
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Informazioni su questo articolo
Form:
liquid
Mol wt:
~49 kDa
Prezzi e disponibilità al momento non sono disponibili
Servizio Tecnico
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Permettici di aiutartiGeneral description
Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.
Application
Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8[1] and Drosophila melanogaster transcription factor interactome.
Learn more product details in our FLAG® application portal.
Physical form
Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
Other Notes
Control protein
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC
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Questo articolo | |||
|---|---|---|---|
| mol wt ~49 kDa | mol wt ~49 kDa | mol wt 1012.97 Da | mol wt ~49 kDa |
| form liquid | form liquid | form lyophilized powder | form - |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Protocolli
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
Protokoll für die Immunpräzipitation (IP) von FLAG-Fusionsproteinen unter Einsatz des monoklonalen M2-Antikörper-Affinitätsgels (4 % Agarose)
Articoli
Vergleich von Elutionstechniken für die Proteinaufreinigung von FLAG® Tag Proteinen im kleinen Maßstab mittels anti-FLAG® M2 Magnetperlen.
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Contenuto correlato
Techniques, réactifs et protocoles permettant de purifier les protéines recombinantes par différentes méthodes telles que la chromatographie d'échange d'ions, la chromatographie d'exclusion stérique ou la chromatographie d'affinité.
Technologies d'expression de protéines destinées à différents systèmes d'expression répondant aux besoins de la recherche et de la production d'agents thérapeutiques et de vaccins.
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| P7582-100UG | 04061834395362 |
| P7582-50UG | 04061833266069 |
| P7582-40UG | 04061833266052 |
| P7582-25UG | 04061833266045 |
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Does the FLAG-BAP fusion protein also contain a his tag?
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