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P1709

Peroxidase, Maleimide Activated from horseradish

lyophilized powder, >200 units/mg protein

Sinonimo/i:

Maleimide activated HRP

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A voi/SKUDisponibilitàPrezzo
2 mg
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CHF 216.00
3 x 2 mg
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CHF 409.00

Informazioni su questo articolo

UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00284131
Specific activity:
>200 units/mg protein
Biological source:
horseradish

CHF 216.00

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biological source

horseradish

Quality Segment

200

form

lyophilized powder

specific activity

>200 units/mg protein

composition

Protein, ~50% E1%/403

extent of labeling

1-3 mol maleimide per mol protein

storage temp.

−20°C

General description

Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms.

Application

For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent.
Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. [1] This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase. [2]
To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification. [3]

Biochem/physiol Actions

Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.

Physical form

Lyophilized powder containing trehalose and sodium citrate

Other Notes

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20°C.
Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester.

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11428861001P208810108090001
Peroxidase, Maleimide Activated from horseradish lyophilized powder, >200 units/mg protein

Sigma-Aldrich

P1709

Peroxidase, Maleimide Activated from horseradish

Peroxidase (POD), activated from horseradish

Roche

11428861001

Peroxidase (POD), activated

Perossidasi Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

Sigma-Aldrich

P2088

Perossidasi

Peroxidase (POD) grade I, from horseradish

Roche

10108090001

Peroxidase (POD)

specific activity

>200 units/mg protein

specific activity

800 units/mg protein (at 25 °C with ABTS and H2O2 as substrate (pH 5.0).)

specific activity

200-300 units/mg solid (using pyrogallol)

specific activity

≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.), 250 U/mg

biological source

horseradish

biological source

horseradish

biological source

horseradish

biological source

horseradish

form

lyophilized powder

form

lyophilized

form

essentially salt-free, lyophilized powder

form

lyophilized (salt-free)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

-

Quality Level

300

Quality Level

-

extent of labeling

1-3 mol maleimide per mol protein

extent of labeling

-

extent of labeling

-

extent of labeling

-

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number

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