L6533
Lectin from Dolichos biflorus (horse gram)
biotin conjugate, buffered aqueous solution
Sinonimo/i:
Dolichos biflorus agglutinin, DBA
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 5 mg | Per conoscere la disponibilità, visualizza il carrello | CHF 475.00 |
Informazioni su questo articolo
NACRES:
NA.32
UNSPSC Code:
12352202
CHF 475.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutarticonjugate
biotin conjugate
Quality Level
form
buffered aqueous solution
composition
Protein, ≥2.5 mg/mL biuret
extent of labeling
≥3 mol biotin per mol lectin
storage temp.
−20°C
General description
Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.
Application
Lectin from Dolichos biflorus (horse gram) has been used:
- to characterize mouse uterine natural killer (uNK) cells
- in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
- as a species-specific gonocyte marker for the determination of donor cell colonization
- to label undifferentiated spermatogonia/cat testicular tissue
Biochem/physiol Actions
Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Packaging
Sold on the basis of mg active conjugate.
Physical form
contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
1 of 1
Questo articolo | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form buffered aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| extent of labeling ≥3 mol biotin per mol lectin | extent of labeling - | extent of labeling ~2 mol FITC per mol protein | extent of labeling 2-4 mol biotin per mol protein |
| composition Protein, ≥2.5 mg/mL biuret | composition - | composition Protein, ~50% Lowry | composition - |
| conjugate biotin conjugate | conjugate - | conjugate FITC conjugate | conjugate biotin conjugate |
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
B Anne Croy et al.
Methods in molecular biology (Clifton, N.J.), 612, 465-503 (2009-12-25)
The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment
Rajesh Dash et al.
JACC. Cardiovascular interventions, 11(2), 195-205 (2018-01-20)
The study investigated whether a dose response exists between myocardial salvage and the depth of therapeutic hypothermia. Cardiac protection from mild hypothermia during acute myocardial infarction (AMI) has yielded equivocal clinical trial results. Rapid, deeper hypothermia may improve myocardial salvage.
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| L6533-5MG | 04061833263525 |