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F4383

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

≥98%

Sinonimo/i:

4-Fluoro-7-sulfobenzofurazan ammonium salt, 7-Fluorobenz-2,1,3-oxadiazole-4-sulfonic acid ammonium salt, Ammonium 7-fluorobenzofurazan-4-sulfonate, SBD-F

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Cambia visualizzazione
Taglio della confezioneSKUDisponibilitàPrezzo
25 mg
Per conoscere la disponibilità, visualizza il carrello
CHF 599.00
10 mg
Per conoscere la disponibilità, visualizza il carrello
CHF 606.00

Informazioni su questo articolo

Formula condensata:
C6H3FN2O4S · NH3
Numero CAS:
84806-27-9
Peso molecolare:
235.19
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
24894861
MDL number:
MFCD02682109
Beilstein/REAXYS Number:
5199564
Assay:
≥98%

CHF 599.00

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Quality Segment

200

assay

≥98%

storage condition

protect from light

fluorescence

λex 380 nm; λem 515 nm

storage temp.

−20°C

SMILES string

N.OS(=O)(=O)c1ccc(F)c2nonc12

InChI

1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3

InChI key

JXLHNMVSKXFWAO-UHFFFAOYSA-N

General description

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt, also known as SBD-F, is a low molecular weight sensitive fluorescent probe. SBD-F reacts with sulfhydryl groups (disulfides do not react with SBD-F and must first be reduced to thiols, such as tributylphosphine) to produce highly fluorescent compounds. The rate of reaction of thiols with SBD-F gradually increases with increasing pH. High fluorescence intensities are observed at pH 2-12. SBD-F has an excitation range between 380-385nm and an emission range between 510-515nm.

Application

Fluorescent probe for thiols and pre-column labeling of biological thiols for HPLC
SBD-F is suitable for the HPLC determination of biological thiols at the picomole level. SBD-F has been used in HPLC methods for measuring total plasma and serum homocysteine levels. It is also suitable to determine metallothionein in a tandem column HPLC method with an isocratic solvent system. SBD-F has been used for determining the position of disulfide linkages of cysteine residues in proteins and for detecting cysteine-containing peptides.

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Questo articolo
46640F3639C1154
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt ≥98%

Sigma-Aldrich

F4383

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt derivatization grade (HPLC), LiChropur™, ≥98.5% (HPLC)

Supelco

46640

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

4-Fluoro-7-sulfamoylbenzofurazan

Sigma-Aldrich

F3639

4-Fluoro-7-sulfamoylbenzofurazan

4-Chloro-7-sulfobenzofurazan ammonium salt

Sigma-Aldrich

C1154

4-Chloro-7-sulfobenzofurazan ammonium salt

assay

≥98%

assay

≥98.5% (HPLC)

assay

-

assay

>98%

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

room temp

fluorescence

λex 380 nm; λem 515 nm

fluorescence

λex 385 nm; λem 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione)

fluorescence

-

fluorescence

λex 360 nm; λem 410 nm (pH 8.5), λex 383 nm; λem 528 nm (Reaction product)

storage condition

protect from light

storage condition

-

storage condition

-

storage condition

-

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Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000552990
0000554872
0000552990
0000554872
0000518337

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Contenuto correlato

Product Information Sheet - F4383

Product Information Sheet

N P Dudman et al.
Clinical chemistry, 42(12), 2028-2032 (1996-12-01)
The recognition of homocysteine as a vascular risk factor has led to increased clinical interest in assaying plasma homocysteine concentrations. Our aim was to improve the reliability of a widely used assay based on HPLC of the fluorescent 7-benzo-2-oxa-1, 3-diazole-4-sulfonic
T Toyo'oka et al.
Biomedical chromatography : BMC, 3(4), 166-172 (1989-07-01)
Biological thiols and disulfides in rat and hamster tissues were simultaneously determined by HPLC-fluorescence detection using 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) and ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). The coefficients of variation (CV) of the method for reduced glutathione (GSH) and oxidized glutathione (GSSG) in liver
B L Ling et al.
Journal of pharmaceutical and biomedical analysis, 10(10-12), 985-988 (1992-10-01)
The present investigation analyses the potentials of capillary chromatography using packed fused silica capillaries filled with 5 microns RP-18 for the fluorescence determination of glutathione in human blood samples. Adaptation of conventional HPLC equipment for miniaturized chromatographic assays proved successful.
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Numero articolo commerciale globale

SKUGTIN
F4383-1MG04061833617700
F4383-25MG04061833617717
F4383-10MG04061832534688

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