C9282

Colato di Sodio

BioReagent, suitable for cell culture

• Sinonimo/i: Acido 3α,7α,12α-triidrossi-5β-colan-24-oico, Acido colalico
• Formula empirica (notazione di Hill): C₂₄H₃₉NaO₅ · xH₂O
• Numero CAS: 206986-87-0
• Peso molecolare: 430.55 (anhydrous basis)
• Beilstein: 3582354
• Numero CE: 206-643-5
• Numero MDL: MFCD00150749
• Codice UNSPSC: 12161902
• ID PubChem: 24893147
• NACRES: NA.75

Proprietà

• Origine biologica: bovine bile; ovine bile
• Descrizione: anionic
• Nome Commerciale: BioReagent
• Saggio: ≥99.0% (HPLC)
• Stato: powder
• PM: micellar avg mol wt 900-1300
• Numero d'aggregazione: 2-3
• tecniche: cell culture | mammalian: suitable
• CMC: 9-15 mM (20-25°C)
• Solubilità: water: 100 mg/mL, clear, colorless to light yellow
• HLB: 18
• Stringa SMILE: O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1; MUVVIYFKOVLQHL-RCVKHMDESA-M

Descrizione

Descrizione generale

Cholate is a primary bile acid[1] produced from cholesterol in the liver.[2] It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).[3]

Applicazioni

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system[4],
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor[5]

Azioni biochim/fisiol

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.[2]

Informazioni sulla sicurezza

• Indicazioni di pericolo: H412
• Consigli di prudenza: P273 - P501
• Classi di pericolo: Aquatic Chronic 3
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)