C4963
Catalase−polyethylene glycol
lyophilized powder, ~40,000 units/mg protein
Sinonimo/i:
PEG-Catalase
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About This Item
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
Attività specifica
~40,000 units/mg protein
PM
PEG 5,000
Composizione
Protein, ~50% E405
Grado di funzionalizzazione
~40 mol PEG per mol protein
Gruppi immobilizzati alla matrice
secondary amine linkage.
Temperatura di conservazione
−20°C
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Applicazioni
Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.
Azioni biochim/fisiol
Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators.
Confezionamento
Package size based on protein content
Altre note
Catalase from bovine liver coupled to methoxy-polyethylene glycol.
Stato fisico
Contains PEG plus 5% citrate buffer salts
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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