C3868

Anti-Calsequestrin-2

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-CASQ2, Anti-fast-twitch, cardiac muscle Calsequestrin
• Codice UNSPSC: 12352203
• NACRES: NA.44

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen ~55 kDa
• Reattività contro le specie: rat, human
• Reattività contro le specie (prevista in base all’omologia): mouse
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• Concentrazione: ~1.0 mg/mL
• tecniche: western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2; western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)
• N° accesso UniProt: O14958
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CASQ2(845) ; mouse ... Casq2(12373) ; rat ... Casq2(29209)

Descrizione

Descrizione generale

Calsequestrin is a major Ca²⁺ binding protein in cardiac and skeletal muscle.

Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.

Immunogeno

synthetic peptide corresponding to amino acids 57-74 of rat calsequestrin-2, conjugated to KLH. This sequence is highly conserved in human and rat calsequestrin-2 (single amino acid substitution). This sequence is not found in calsequestrin-1.

Applicazioni

Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.

Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)

Azioni biochim/fisiol

Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the sarcoplasmic reticulum (SR).

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)