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A8419

Sigma-Aldrich

Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Goat Anti-Human IgG (gamma-chain specific) HRP

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.77

Origine biologica

goat

Coniugato

peroxidase conjugate

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

tecniche

direct ELISA: 1:40,000
dot blot: 1:100,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Categorie correlate

Descrizione generale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The specificity of Anti-Human IgG (γ-chain specific)-Peroxidase antibody is determined by immunoelectrophoresis and Ouchterlony Double Diffusion techniques. This antibody is specific for for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones lambda myeloma proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.

Specificità

This antibody is specific for for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones lambda myeloma proteins.

Immunogeno

Human IgG

Applicazioni

Anti-Human IgG (γ-chain specific) Peroxidase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and western blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
IgM concentration in sera from patients with chronic CA syndrome was determined by ELISA using HRP-conjugated goat anti-human (mu chain specific) as the secondary antibody.

Azioni biochim/fisiol

Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Nota sulla preparazione

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Qingbo Liu et al.
Journal of virology, 95(12) (2021-04-09)
Broadly neutralizing antibodies (bNAbs) are the focus of increasing interest for human immunodeficiency virus type 1 (HIV-1) prevention and treatment. Although several bNAbs are already under clinical evaluation, the development of antibodies with even greater potency and breadth remains a
Heriberto Caballero-Ortega et al.
Journal of infection in developing countries, 8(5), 642-647 (2014-05-14)
There are few articles on evaluation of Toxoplasma gondii serological tests. Besides, commercially available tests are not always useful and are expensive for studies in open population. The aim of this study was to evaluate in-house ELISA and western blot
An accessory protease inhibitor to increase the yield and quality of a tumour-targeting mAb in Nicotiana benthamiana leaves
Jutras PV, et al.
Testing, 11(11), e0167086-e0167086 (2016)
Qingbo Liu et al.
Nature communications, 10(1), 721-721 (2019-02-15)
Broadly neutralizing antibodies (bNAbs) represent a promising alternative to antiretroviral drugs for HIV-1 prevention and treatment. Selected antibodies to the CD4-binding site bolster envelope trimer binding via quaternary contacts. Here, we rationally engraft a new paratope, i.e., the extended heavy-chain
Philippe V Jutras et al.
PloS one, 11(11), e0167086-e0167086 (2016-11-29)
The overall quality of recombinant IgG antibodies in plants is dramatically compromised by host endogenous proteases. Different approaches have been developed to reduce the impact of endogenous proteolysis on IgGs, notably involving site-directed mutagenesis to eliminate protease-susceptible sites or the

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