A7189
L-Alanine Dehydrogenase from Bacillus subtilis
ammonium sulfate suspension, ≥20 units/mg protein (Lowry)
Sinonimo/i:
L-Alanine: NAD+ oxidoreductase (deaminating)
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Cambia visualizzazione
| Taglio della confezione | SKU | Disponibilità | Prezzo |
|---|---|---|---|
| 250 units | Per conoscere la disponibilità, visualizza il carrello | CHF 425.00 | |
| 500 units | Per conoscere la disponibilità, visualizza il carrello | CHF 828.00 |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-847-9
MDL number:
Specific activity:
≥20 units/mg protein (Lowry)
Biological source:
Bacillus subtilis
CHF 425.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartibiological source
Bacillus subtilis
Quality Level
form
ammonium sulfate suspension
specific activity
≥20 units/mg protein (Lowry)
storage temp.
2-8°C
General description
L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain.
Application
L-Alanine Dehydrogenase from Bacillus subtilis has been used in the carbon nanotube columns for H2-driven biocatalysis hydrogenation studies.
L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection [1].
Biochem/physiol Actions
L-Alanine Dehydrogenase is essential for sporulation in Bacillus subtilis.
L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order.[1] Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.[2]
Physical form
Suspension in 2.4 M (NH4)2SO4 solution, pH 7.0
Other Notes
One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.
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Questo articolo | |||
|---|---|---|---|
| specific activity ≥20 units/mg protein (Lowry) | specific activity ~30 units/mg protein (Lowry) | specific activity ≥250 units/mg protein (biuret) | specific activity ≥60 units/mg protein |
| biological source Bacillus subtilis | biological source Bacillus subtilis | biological source - | biological source - |
| form ammonium sulfate suspension | form buffered aqueous glycerol solution | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Articoli
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Contenuto correlato
H 2-Driven biocatalytic hydrogenation in continuous flow using enzyme-modified carbon nanotube columns
Zor C, et al.
Chemical Communications (Cambridge, England), 53(71), 9839-9841 (2017)
Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.
Siranosian K, et al.
Journal of Bacteriology, 175(21), 6789-6796 (1993)
Domain motions and functionally-key residues of l-alanine dehydrogenase revealed by an elastic network model
Li XY, et al.
International Journal of Molecular Sciences, 16(12), 29383-29397 (2015)
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| A7189-250UN | 04061838168511 |
| A7189-500UN | 04061838168528 |