A7080
Amphiregulin human
recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
Sinonimo/i:
AREG, CRDGF, SDGF, AR
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About This Item
Prodotti consigliati
Origine biologica
human
Livello qualitativo
Ricombinante
expressed in E. coli
Saggio
≥97% (SDS-PAGE)
Forma fisica
lyophilized powder
Potenza
10-100 ng/mL ED50/EC50
PM
~11 kDa
Confezionamento
pkg of 1 mg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... AREG(374)
Azioni biochim/fisiol
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
Componenti
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
Avvertenza
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
Stato fisico
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
Nota sulla preparazione
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
Risultati analitici
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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