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A6410

Apirasi

Name: Apirasi
Brand: SIGMA
Price: 170 CHF

ATPase ≥200 units/mg protein, lyophilized powder

Sinonimo/i:

Adenosina 5′-difosfatasi, Adenosina 5′-trifosfatasi

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Taglio della confezione	Disponibilità	Prezzo
100 units	Per conoscere la disponibilità, visualizza il carrello	CHF 170.00
200 units	Per conoscere la disponibilità, visualizza il carrello	CHF 237.00
500 units	Per conoscere la disponibilità, visualizza il carrello	CHF 573.00

Informazioni su questo articolo

Numero CAS:

9000-95-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-569-8

MDL number:

MFCD00130542

Numero CE:

3.6.1.5 (BRENDA, IUBMB)

CHF 170.00

Per conoscere la disponibilità, visualizza il carrello

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Proprietà

type

Grade VI

Quality Segment

200

form

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≤15 % of base activity ADPase

mol wt

~49 kDa by gel filtration

composition

Protein, 30-60%

solubility

H₂O: soluble 1.0 mg/mL

foreign activity

Acid Phosphatase ≤2% of base activity

storage temp.

−20°C

Descrizione

General description

Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.

Application

Apyrase from potatoes has been used:

to deplete ATP
to degrade endogenous extracellular ATP
as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1].

In diverse varietà di S. tuberosum si trovano almeno due isoenzimi: uno con un rapporto ATPasi/ADPasi elevato (∼10) e un altro con un rapporto basso (∼1).
Reazione: ATP → ADP+P_i → AMP+2P_i.

Biochem/physiol Actions

Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from white potato

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

This grade has an exceptionally high ATPase/ADPase ratio.

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Questo articolo	A7646	SRE0019	A6535
Sigma-Aldrich A6410 Apyrase from potatoes	Sigma-Aldrich A7646 Apyrase from potatoes	Sigma-Aldrich SRE0019 Apyrase from potatoes	Sigma-Aldrich A6535 Apyrase from potatoes
form lyophilized powder	form lyophilized powder (partially purified)	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt ~49 kDa by gel filtration	mol wt ~49 kDa by gel filtration	mol wt -	mol wt -
solubility H₂O: soluble 1.0 mg/mL	solubility H₂O: soluble 10 mg/mL, slightly hazy	solubility H₂O: soluble 1.0 mg/mL	solubility H₂O: soluble 1.0 mg/mL
Quality Level 200	Quality Level 200	Quality Level 400	Quality Level 300
foreign activity Acid Phosphatase ≤2% of base activity	foreign activity Acid Phosphatase ≤5% of base activity	foreign activity Acid Phosphatase ≤2% of base activity	foreign activity Acid Phosphatase ≤2% of base activity