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A3687

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Sinonimo/i:

Alkaline Phosphatase Secondary Antibody, Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, Anti Rabbit Sigma, anti rabbit antibody

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0.25 ML
CHF 103.00
0.5 ML
CHF 184.00
1 ML
CHF 248.00

CHF 103.00

Spedizione prevista il22 maggio 2025

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Cambia visualizzazione
0.25 ML
CHF 103.00
0.5 ML
CHF 184.00
1 ML
CHF 248.00

About This Item

Numero MDL:
MFCD00162782
Codice UNSPSC:
12352203
NACRES:
NA.46

CHF 103.00

Spedizione prevista il22 maggio 2025

Richiedi un ordine bulk

Origine biologica

goat

Livello qualitativo

200

Coniugato

alkaline phosphatase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

buffered aqueous glycerol solution

Reattività contro le specie

rabbit

tecniche

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

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Categorie correlate

Descrizione generale

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds all rabbit Igs.

Immunogeno

purified rabbit IgG as the immunogen

Applicazioni

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Dot blot (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (10 papers)
Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for western blot and ELISA applications.the antibody can also be used for immunohistochemistry (at 1:50, using formalin-fixed, paraffin-embedded sections).
The level of hypoxyprobe binding proteins present in retinal mouse tissue was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary at a dilution of 1:2000 in 5% tween/PBS. Substrate used was 4-nitrophenyl phosphate disodium salt hexahydrate (Sigma).

Stato fisico

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

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Certificati d'analisi (COA)

Lot/Batch Number
0000423167
0000423167
0000343830
0000343831
0000343871

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Sônia de Fátima Soto et al.
PloS one, 12(8), e0183314-e0183314 (2017-08-19)
Female Wistar rats were exposed to filtered air (F) or to concentrated fine particulate matter (P) for 15 days. After mating, the rats were divided into four groups and again exposed to F or P (FF, FP, PF, PP) beginning
Tanyth E de Gooyer et al.
Investigative ophthalmology & visual science, 47(12), 5553-5560 (2006-11-24)
This study was conducted to evaluate whether regions of the retinal neuropile become hypoxic during periods of high oxygen consumption and whether depletion of the outer retina reduces hypoxia and related changes in gene expression. Retinas from rhodopsin knockout (Rho-/-)
Pingxi Xiao et al.
Experimental and therapeutic medicine, 14(4), 3812-3816 (2017-10-19)
Rheumatic heart disease (RHD) occurs due to the accumulation of complications associated with rheumatic fever, and it results in high morbidity and mortality. The majority of cases of RHD are diagnosed in the chronic stages, when treatment options are limited.
Alexander S Dowdell et al.
Journal of bacteriology, 199(6) (2017-01-11)
The Lyme disease spirochete
Mark A Russell et al.
Islets, 5(2), 95-105 (2013-03-21)
Pro-inflammatory cytokines are important mediators of β-cell demise in type 1 diabetes, and similar mechanisms are increasingly implicated in type 2 diabetes, where a state of chronic inflammation may persist. It is likely that the actions of anti-inflammatory cytokines are
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