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Merck

A2189

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal

clone MB-11, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase

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0.2 ML

CHF 229.00

CHF 229.00


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Informazioni su questo articolo

NACRES:
NA.77
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
MB-11, monoclonal
Application:
DB, ELISA (d)
Citations:
9

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biological source

mouse

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

MB-11, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:35,000, dot blot: 1:20,000 using 20 ng of human IgM/dot, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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Questo articolo
A3437A3076A9794
antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

-

technique(s)

direct ELISA: 1:35,000, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence), dot blot: 1:20,000 using 20 ng of human IgM/dot

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

technique(s)

direct ELISA: 1:50,000, dot blot: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:60,000

biological source

mouse

biological source

goat

biological source

mouse

biological source

goat

isotype

IgG2b

isotype

-

isotype

IgG2b

isotype

-

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. IgMs have been implicated in CNS myelin repair and increased secretory IgM levels have been linked to rhino-conjunctivitis . Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM. The product does not react with human IgG, IgA or light chains.
Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Human IgM isolated from pooled normal human serum

Application

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody purified from hybridoma cell culture has been used in immunocytochemistry and enzyme linked immunosorbent assay (ELISA)

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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A natural human IgM that binds to gangliosides is therapeutic in murine models of amyotrophic lateral sclerosis
Xu X, et al.
Disease models & mechanisms, 8(8), 831-842 (2015)
Ines Pree et al.
Journal of immunology (Baltimore, Md. : 1950), 179(8), 5309-5316 (2007-10-04)
Previously, we have constructed recombinant derivatives of the major birch pollen allergen, Bet v 1, with a more than 100-fold reduced ability to induce IgE-mediated allergic reactions. These derivatives differed from each other because the two recombinant Bet v 1
Johan Bours et al.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 243(5), 456-463 (2005-06-03)
With various methods, secretory immunoglobulin M (sIgM) was assessed in tears of patients with rhino-conjunctivitis. Tears were analyzed by microimmunoelectrophoresis (MIE), size-exclusion high-pressure liquid chromatography (SE-HPLC), sodium dodecyl sulphate (SDS) electrophoresis and isoelectric focusing. Only very small traces of serum
Ana Luísa Tomás et al.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 39(11), 2205-2209 (2020-06-20)
Interest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Results
Sina Sarikhani et al.
Applied biochemistry and biotechnology, 162(5), 1249-1257 (2010-02-18)
As IgM is the first isotype of antibody which appears in blood after initial exposure to a foreign antigen in the pattern of primary response, detection, and quantification of this molecule in blood seems invaluable. To approach these goals, generation

Numero articolo commerciale globale

SKUGTIN
A2189-.2ML04061838352149

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