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A2189

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal

clone MB-11, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase

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0.2 mL
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CHF 229.00

Informazioni su questo articolo

NACRES:
NA.77
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
MB-11, monoclonal
Application:
DB, ELISA (d)
Citations:
9

CHF 229.00


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biological source

mouse

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

MB-11, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:35,000, dot blot: 1:20,000 using 20 ng of human IgM/dot, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. IgMs have been implicated in CNS myelin repair and increased secretory IgM levels have been linked to rhino-conjunctivitis . Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM. The product does not react with human IgG, IgA or light chains.
Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Human IgM isolated from pooled normal human serum

Application

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody purified from hybridoma cell culture has been used in immunocytochemistry and enzyme linked immunosorbent assay (ELISA)

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo
A2064A3437A3076
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

direct ELISA: 1:35,000, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence), dot blot: 1:20,000 using 20 ng of human IgM/dot

technique(s)

direct ELISA: 1:50,000, dot blot: 1:80,000, immunohistochemistry: suitable, western blot (chemiluminescent): 1:80,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

biological source

mouse

biological source

mouse

biological source

goat

biological source

mouse

isotype

IgG2b

isotype

IgG1

isotype

-

isotype

IgG2b


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Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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A natural human IgM that binds to gangliosides is therapeutic in murine models of amyotrophic lateral sclerosis
Xu X, et al.
Disease models & mechanisms, 8(8), 831-842 (2015)
Ines Pree et al.
Journal of immunology (Baltimore, Md. : 1950), 179(8), 5309-5316 (2007-10-04)
Previously, we have constructed recombinant derivatives of the major birch pollen allergen, Bet v 1, with a more than 100-fold reduced ability to induce IgE-mediated allergic reactions. These derivatives differed from each other because the two recombinant Bet v 1
Johan Bours et al.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 243(5), 456-463 (2005-06-03)
With various methods, secretory immunoglobulin M (sIgM) was assessed in tears of patients with rhino-conjunctivitis. Tears were analyzed by microimmunoelectrophoresis (MIE), size-exclusion high-pressure liquid chromatography (SE-HPLC), sodium dodecyl sulphate (SDS) electrophoresis and isoelectric focusing. Only very small traces of serum



Numero articolo commerciale globale

SKUGTIN
A2189-.2ML04061838352149

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