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A1346

Sigma-Aldrich

ASB-14

Sinonimo/i:

3-[N,N-Dimethyl(3-myristoylaminopropyl)ammonio]propanesulfonate, Amidosulfobetaine-14

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About This Item

Formula empirica (notazione di Hill):
C22H46N2O4S
Numero CAS:
216667-08-2
Peso molecolare:
434.68
Numero MDL:
MFCD03456122
Codice UNSPSC:
12161902
NACRES:
NA.28

Descrizione

zwitterionic

Saggio

≥95.0% (HPLC)

Forma fisica

powder

PM

average mol wt >10,000

Numero d'aggregazione

≥23

Temperatura di conservazione

2-8°C

InChI

1S/C22H46N2O4S/c1-4-5-6-7-8-9-10-11-12-13-14-17-22(25)23-18-15-19-24(2,3)20-16-21-29(26,27)28/h4-21H2,1-3H3,(H-,23,25,26,27,28)
UTSXERRKRAEDOV-UHFFFAOYSA-N

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Descrizione generale

ASB-14 comprises alkyl tails and amidosulfobetaine headgroup.

Applicazioni

ASB-14 has been used as a component of rehydration buffer for proteomic sample preparation from Leishmania donovani.
Zwitterionic detergent. Useful for solubilization of proteins, including membrane proteins, for 2D electrophoresis.

Azioni biochim/fisiol

ASB-14 is useful in proteasome extraction especially for the membrane associated proteins. Along with CHAPS (3-(3-cholamidopropyl)dimethylammonio)-1-propanesulfonate), it is employed in protein solubilization even in the presence of thiourea.

Risultati analitici

ASB-14 (amidosulfobetaine-14), a membrane solubilizing zwitterionic detergent (medium chain amidosulfobetaine surfactant), may be used in protein and macromolecule research for the preparative extraction and solubilization of proteins for separation and analysis by methods such as 2D-electrophoresis. ASB-14 may be used in tissue engineering for antigen removal (AR) for xenogeneic scaffold generation. ASB-14 may be used to develop and study zwitterionic micelles.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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BICINE ≥99% (titration)

Sigma-Aldrich

B3876

BICINE

ASB-14 A zwitterionic amidosulfobetaine detergent useful for solubilizing proteins visualized by 2D-electrophoresis.

Millipore

182750

ASB-14

ASB-16 A zwitterionic amidosulfobetaine detergent useful for solubilizing proteins visualized by 2D-electrophoresis.

Millipore

182755

ASB-16

Nityakalyani Srinivas et al.
Science (New York, N.Y.), 327(5968), 1010-1013 (2010-02-20)
Antibiotics with new mechanisms of action are urgently required to combat the growing health threat posed by resistant pathogenic microorganisms. We synthesized a family of peptidomimetic antibiotics based on the antimicrobial peptide protegrin I. Several rounds of optimization gave a
Protein Sample Preparation for Proteomic Analysis in Leishmania donovani
Alexandros A and Despina S
Bio-protocol, 4(5), 70-75 (2014)
Francisella t ularensis Proteome: Low Levels of ASB-14 Facilitate the Visualization of Membrane Proteins in Total Protein Extracts
Twine SM, et al.
Journal of Proteome Research, 4(5), 1848-1854 (2005)
Maelene L Wong et al.
Acta biomaterialia, 9(5), 6492-6501 (2013-01-17)
The ability of residual antigens on decellularized tissue to elicit the immune response upon implantation motivates development of a more rigorous antigen removal (AR) process for xenogeneic scaffold generation. Antigen removal strategies promoting solubilization of hydrophilic proteins (predominantly cytoplasmic) enhance
Zhiquan Chen et al.
Free radical biology & medicine, 102, 77-86 (2016-11-15)
Nrf2/ARE signaling pathway is a crucial cellular defense system to cope with oxidative stress, which is adaptively activated, in diabetic condition that is not efficient enough to resist the oxidative stress provoked by hyperglycemia. We have previously demonstrated that Connexin43
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