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A0144

Sigma-Aldrich

Adenosine 3′,5′-cyclic monophosphate–Agarose

lyophilized powder

Sinonimo/i:

3′,5′-cAMP agarose

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About This Item

Numero MDL:
Codice UNSPSC:
41106500
eCl@ss:
32160414
NACRES:
NA.56

Forma fisica

lyophilized powder

Grado di funzionalizzazione

≥2 μmol per mL

Matrice

4% beaded agarose

Attivazione matrice

cyanogen bromide

Gruppi immobilizzati alla matrice

C-8

Braccio spaziatore

9 atoms

Temperatura di conservazione

−20°C

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Applicazioni

Adenosine 3′,5′-cyclic monophosphate-Agarose (3′,5′-cAMP agarose) may be used in affinity chromatography for the separation of the subunits of cAMP-dependent protein kinase or for the purification of the cAMP receptor subunit. Research on schizophrenia has shown that abnormalities in the cAMP signaling pathway, may contribute to the pathophysiology of the disorder.

Stato fisico

Lyophilized powder stabilized with lactose

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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M Meyer et al.
Journal of bacteriology, 183(18), 5248-5256 (2001-08-22)
Klebsiella pneumoniae is able to grow anaerobically with citrate as a sole carbon and energy source by a fermentative pathway involving the Na(+)-dependent citrate carrier CitS, citrate lyase, and oxaloacetate decarboxylase. The corresponding genes are organized in the divergent citC
Florencia Kronberg et al.
Archives of biochemistry and biophysics, 509(1), 66-75 (2011-03-09)
cAMP-dependent protein kinase (PKA) catalytic (C) and regulatory (R) subunits from Yarrowia lipolytica are encoded by single genes, TPK1 and RKA1, respectively. Here we performed the heterologous expression, purification and characterization of the R subunit from Y. lipolytica yeast cells
Ganesh Anand et al.
Biochemistry, 46(32), 9283-9291 (2007-07-31)
To better understand the molecular mechanism of cAMP-induced and substrate-enhanced activation of type-I A-kinase, we measured the kinetics of A-kinase regulatory subunit interactions using a stopped-flow spectrofluorometric method. Specifically, we conjugated fluorescein maleimide (FM) to two separate single cysteine-substituted and
Adam J Funk et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 37(4), 896-905 (2011-11-04)
Recent evidence suggests that schizophrenia may result from alterations of integration of signaling mediated by multiple neurotransmitter systems. Abnormalities of associated intracellular signaling pathways may contribute to the pathophysiology of schizophrenia. Proteins and phospho-proteins comprising mitogen activated protein kinase (MAPK)
Purification of rabbit skeletal muscle protein kinase regulatory subunit using cyclic adenosine-3':5'-monophosphate affinity chromatography.
W L Dills et al.
Biochemical and biophysical research communications, 62(1), 70-77 (1975-01-06)

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