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Sigma-Aldrich

Fast Blue BB Salt hemi(zinc chloride) salt

for microscopy (Hist.)

Sinonimo/i:

4-Amino-2,5-diethoxybenzanilide diazotated zinc double salt, 4-Benzoylamino-2,5-diethoxybenzenediazonium chloride hemi(zinc chloride) salt

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About This Item

Formula condensata:
C17H18N3O3Cl · 1/2 ZnCl2
Numero CAS:
5486-84-0
Peso molecolare:
415.94
Colour Index:
37175
Beilstein:
1845288
Numero CE:
226-817-4
Numero MDL:
MFCD00074765
Codice UNSPSC:
12171500
ID PubChem:
57650235
NACRES:
NA.47

Grado

for microscopy (Hist.)

Livello qualitativo

100

Forma fisica

powder

Punto di fusione

157 °C

Solubilità

hot water: 0.01 g/10 mL

εmax

≥100 at 390-400 nm in water
≥200 at 325-335 nm in water

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Cl-].[Cl-].Cl[Zn]Cl.CCOc1cc([N+]#N)c(OCC)cc1NC(=O)c2ccccc2.CCOc3cc([N+]#N)c(OCC)cc3NC(=O)c4ccccc4

InChI

1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2
CMFRFQODFZBKTI-UHFFFAOYSA-L

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Descrizione generale

Fast blue BB salt hemi(zinc chloride) salt is suitable as a common coloring agent for TLC (thin layer chromatography) analysis of cannabis. It is also suitable for histochemistry studies. It is of 415.9kDa.

Applicazioni

Fast blue BB salt hemi(zinc chloride) salt has been used to prepare substrate solution for tissue-blot enzyme-immunoassay procedure. It has been used in immunohistochemical staining.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H302

Consigli di prudenza

P301 + P312 + P330

Classi di pericolo

Acute Tox. 4 Oral

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Certificati d'analisi (COA)

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L Wagner et al.
Journal of immunological methods, 142(2), 147-155 (1991-09-13)
A trypsin-like serine esterase (SE) is known to be present in cultured cells with cytolytic potential. The distribution pattern of this enzyme in haematological cells and body tissues has been assessed using a method which permits rapid identification of individual
B Algermissen et al.
Experimental dermatology, 3(6), 290-297 (1994-12-01)
In order to gain insights into the dynamics of mast cell subpopulations in normal and diseased skin, a novel enzyme-histochemical double and triple staining method was employed that allowed the detection of metachromasia (toluidine blue) and the mast cell proteases
Marjorie B Medina
Journal of agricultural and food chemistry, 59(5), 1565-1571 (2011-02-12)
A new method for the detection of phenolics in food systems was developed. This method is based on interactions of phenolics with Fast Blue BB diazonium salt in alkali pH, forming azo complexes, with the absorbance measured at 420 nm
L H Rademakers et al.
The Histochemical journal, 21(5), 301-308 (1989-05-01)
Non-specific esterase (NSE) activity was demonstrated in glutaraldehyde-fixed monolayers of murine peritoneal macrophages. Using 2-naphthylthiol acetate (NTA) as substrate and Fast Blue BB as coupling agent a strong osmiophilic reaction product was obtained. The reaction product was observed as electron-dense
M B Medina et al.
Journal of chromatography, 614(2), 315-323 (1993-05-05)
Clean-up procedures for the isolation of zeranol and diethylstilbestrol (DES) were modified to reduce the analysis time and to increase the efficiency of purification. Several dyes (Fast Blue BB, Fast Corinth V, Fast Blue RR, Fast Blue B, Fast Red
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