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43819

DL-Ditiotreitolo

≥99.0% (RT)

Sinonimo/i:

treo-1,4-dimercapto-2,3-butandiolo, DTT, Reagente di Cleland

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1 g
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CHF 73.10
5 g
Per conoscere la disponibilità, visualizza il carrello
CHF 275.00
25 g
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CHF 1’040.00

Informazioni su questo articolo

Formula condensata:
HSCH2CH(OH)CH(OH)CH2SH
Numero CAS:
Peso molecolare:
154.25
UNSPSC Code:
12352201
NACRES:
NA.21
PubChem Substance ID:
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:

CHF 73.10


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Quality Segment

assay

≥99.0% (RT)

form

powder

reaction suitability

reagent type: reductant

impurities

≤0.3% 4,5-dihydroxy-1,2-dithiane

mp

40-43 °C, 41-44 °C (lit.)

solubility

H2O: 50 mg/mL, clear, colorless, H2O: freely soluble, acetone: freely soluble, chloroform: freely soluble, diethyl ether: freely soluble, ethanol: freely soluble, ethyl acetate: freely soluble

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤250 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

Application

Il DL-ditio-treitolo è un componente centrale nella preparazione del tampone a sua volta utilizzato nella preparazione di citosol delle piastrine.[1] È anche importante nell′isolamento di RNA usando sali di guanidinio e omogeneizzazione di tessuto[2] e cellule[3].
Un reagente eccellente per impedire l′ossidazione di gruppi SH ridotti; riduce i disolfuri quantitativamente. DTT è efficace in soluzioni tampone dei campioni per ridurre i legami proteina disolfuro prima dell′SDS-PAGE. DTT può anche essere usato per ridurre il ponte disolfuro del reticolante N,N′-bis(acriloil)cistammina per scindere la matrice del gel di poliacrilammide. DTT è meno aggressivo e tossico rispetto al 2-mercaptoetanolo. Tipicamente si ricorre a una concentrazione di DTT (100 mM) sette volte più bassa rispetto al 2-mercaptoetanolo (5% v/v, 700 mM).

Biochem/physiol Actions

Il DL-ditio-treitolo denatura le proteine riducendo i legami disolfuro a gruppi SH. Impedisce la formazione intramolecolare e intermolecolare di legami disolfuro fra residui di cisteina di proteine.[4]

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Questo articolo
D0632D916343816
assay

≥99.0% (RT)

assay

≥98% (HPLC), ≥99.0% (titration)

assay

≥99%

assay

900-1100 mmol/L (Titration molarity)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

≤0.3% 4,5-dihydroxy-1,2-dithiane

impurities

-

impurities

-

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

mp

40-43 °C, 41-44 °C (lit.)

mp

41-44 °C (lit.)

mp

40-44 °C, 41-44 °C (lit.)

mp

-

form

powder

form

powder

form

powder

form

liquid


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pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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M B Donati et al.
Cancer research, 46(12 Pt 1), 6471-6474 (1986-12-01)
It has repeatedly been proposed that fibrin plays a role in tumor growth and metastasis. Among tumor cell products or activities which may promote clot formation, cancer procoagulant (CP), a direct activator of coagulation factor X, has been suggested to
R G Knowles et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(13), 5159-5162 (1989-07-01)
A soluble enzyme obtained from rat forebrain catalyzes the NADPH-dependent formation of nitric oxide (NO) and citrulline from L-arginine. The NO formed stimulates the soluble guanylate cyclase and this stimulation is abolished by low concentrations of hemoglobin. The synthesis of
M W Radomski et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(13), 5193-5197 (1990-07-01)
Aggregation of human washed platelets with collagen is accompanied by a concentration-dependent increase in cyclic GMP but not cyclic AMP. NG-Monomethyl-L-arginine (L-MeArg), a selective inhibitor of nitric oxide (NO) synthesis from L-arginine, reduces this increase and enhances aggregation. L-Arginine, which



Numero articolo commerciale globale

SKUGTIN
43819-25G04061832132938
43819-5G04061832132990
43819-1G04061835515509

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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