This solution is prepared based on the unit activity. The protein content is not determined, therefore the weight of the enzyme is not available. Should this information be required, the end user would have to determine the protein concentration by Bradford Assay.
25810
Chloroperoxidase from Caldariomyces fumago
aqueous suspension, brown, >10,000 U/mL
Sinonimo/i:
Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
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1 ML
CHF 398.00
CHF 398.00
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Cambia visualizzazione
1 ML
CHF 398.00
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
CHF 398.00
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Prodotti consigliati
Origine biologica
fungus (Caldariomyces fumago)
Stato
aqueous suspension
Concentrazione
>10,000 U/mL
Colore
brown
Temperatura di conservazione
2-8°C
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Descrizione generale
Applicazioni
A useful alternative to lactoperoxidase for 131I ion labeling studies, for bromination of proteins, and for 36Cl labeling of macromolecules in long-term isolation procedures.
Azioni biochim/fisiol
Definizione di unità
1 U corresponds to the amount of enzyme which converts 1 μmol of monochlorodimedone to dichlorodimedone per minute at pH 2.75 and 25 °C in the presence of KCl and H2O2.
Stato fisico
Supplied as a suspension in 0.1 M sodium phosphate pH 4.0.
Altre note
Oxidation of aminopyrine;citation Chloroperoxidase, a peroxidase with potential;citation Chloroperoxidase-catalyzed asymmetric transformations.citationcitation
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves
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H Sayo et al.
Chemical & pharmaceutical bulletin, 37(12), 3347-3350 (1989-12-01)
Although in the absence of halide ion chloroperoxidase did not catalyze the ethylhydroperoxide (EHP)-supported oxidation of aminopyrine, in the presence of Br- or Cl-, chloroperoxidase did catalyze the oxidation of aminopyrine, generating the aminopyrine cation radical (AP+). The initial rate
M.A. Pickard et al.
Journal of Industrial Microbiology, 7, 235-235 (1991)
Fast and efficient purification of chloroperoxidase from C. fumago
Yazbik V and Ansorge SM
Process. Biochem., 45(2), 279-283 (2010)
H. Fu et al.
The Journal of Organic Chemistry, 57, 7265-7265 (1992)
Daniel Andrew et al.
Biochemical and biophysical research communications, 415(4), 646-649 (2011-11-15)
Azide is a well-known inhibitor of heme-enzymes. Herein, we report the counter-intuitive observation that at some concentration regimes, incorporation of azide in the reaction medium enhances chloroperoxidase (CPO, a heme-enzyme) mediated one-electron abstractions from several substrates. A diffusible azidyl radical
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I would like to know the weight of enzyme in 25810-1ML-F. Thanks!
1 answer-
Helpful?
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Kann ich die CfuCPO-probe auch in Gefrierschrank lagern?
1 answer-
Recommended storage temperature of the product is 2-8°C. Any potential damage resulting from the freeze/thaw process has not been investigated. It is not advised to store the product in the freezer.
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