SCC154

56FHTEo- Human Tracheal Epithelial Cell Line

Human

• Sinonimo/i: 56 FHTE
• UNSPSC Code: 41106514
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: human

Proprietà

• Nome del prodotto: 56FHTEo- Human Tracheal Epithelial Cell Line, 56FHTEo- human tracheal epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.
• biological source: human
• technique(s): cell based assay: suitable, cell culture | mammalian: suitable
• shipped in: ambient
• storage temp.: -140 to -196°C

Descrizione

General description

56FHTEo- is a human fetal tracheal epithelial cell line that is immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) [1]. The cell line expresses the wild-type cystic fibrosis transmembrane conductance regulator (CFTR) and shows cAMP-dependent chloride ion transport and calcium ion-dependent chloride transport [1]. The cell line is permissive for adenoviruses [2].

Application

56FHTEo- human tracheal epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Biochem/physiol Actions

Epithelial Cells

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable