APT110
Apo-Direct TUNEL Assay Kit
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
Sinonimo/i:
Apoptosis detection kit, TUNEL apoptosis assay, TUNEL assay kit
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About This Item
Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Livello qualitativo
Reattività contro le specie
vertebrates
Produttore/marchio commerciale
ApoDIRECT
Chemicon®
tecniche
flow cytometry: suitable
Metodo di rivelazione
fluorometric
Condizioni di spedizione
wet ice
Descrizione generale
The CHEMICON APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
Applicazioni
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.
Componenti
The following components are included with the APO-DIRECT Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.
Positive Control Cells,Brown cap
Negative Control Cells, Natural cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
F-dUTP, Orange cap
Rinsing Buffer, Red cap
PI/RNase Staining Buffer, Amber bottle
Positive Control Cells,Brown cap
Negative Control Cells, Natural cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
F-dUTP, Orange cap
Rinsing Buffer, Red cap
PI/RNase Staining Buffer, Amber bottle
Stoccaggio e stabilità
Precautions and Warnings:
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1
Codice della classe di stoccaggio
3 - Flammable liquids
Punto d’infiammabilità (°F)
69.8 °F
Punto d’infiammabilità (°C)
21 °C
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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