ABE598

Anti-TCF12 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: HTF4, HEB, BHLHB20, Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• N° accesso UniProt: Q99081
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TCF12(6938)

Descrizione

Descrizione generale

TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family. TCF12 is a transcriptional regulator involved in the initiation of neuronal differentiation, and it has been shown to activate transcription by binding to the E box (5′-CANNTG-3′). TCF12 has been shown to interact with PTF1A, NEUROD2 and RUNX1T1. TCF12 is suggested to play a critical role in proper T cell differentiation by blocking the thymocyte proliferation in combination with E2A. Recent studies have shown that TCF12 is also an important factor required for the development of invariant natural killer T cells. TCF12 is expressed in many tissues, and more specifically in skeletal muscle, thymus, B- and T-cells.

Immunogeno

KLH-conjugated linear peptide corresponding to human TCF12.

Applicazioni

Detect TCF12 using this rabbit polyclonal antibody, Anti-TCF12 Antibody validated for use in western blotting, ICC & IHC.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TCF12 in human tonsil tissue.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected TCF12 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TCF12 in 15 µg of HeLa cell lysate.

Descrizione del bersaglio

~83 kDa observed. Uncharacterized bands may appear in some lysates. Uniprot describes 3 isoforms between ~55 kDa and ~76 kDa

Stato fisico

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable