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70922

BugBuster® HT Protein Extraction Reagent

Sinonimo/i:

Cell lysis reagent, Protein extraction buffer

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Taglio della confezioneSKUDisponibilitàPrezzo
100 mL
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CHF 201.00
500 mL
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CHF 710.00
1 L
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CHF 1’230.00

Informazioni su questo articolo

NACRES:
NA.77
UNSPSC Code:
41106511

CHF 201.00


Per conoscere la disponibilità, visualizza il carrello

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form

liquid

Quality Segment

manufacturer/tradename

Novagen®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

2-8°C

General description

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Application

BugBuster® HT Protein Extraction Reagent has been used:
  • to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
  • for protein extraction from bacterial cell pellet
  • used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Features and Benefits

  • Fast protein extraction and nucleic acid degradation
  • Ideal for processing various samples of different volumes
  • Increased extraction is ensured by the addition of rLysozyme solution

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Questo articolo
70584-M7092171186
shipped in

wet ice

shipped in

-

shipped in

ambient

shipped in

wet ice

form

liquid

form

liquid

form

liquid

form

liquid

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

10-30°C

storage temp.

-

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®


Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 2



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Articoli

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Contenuto correlato

E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.

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Numero articolo commerciale globale

SKUGTIN
70922-404055977272765
70922-304055977272758
70922-504055977272192

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