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70899

Ni-NTA Buffer Kit

Sinonimo/i:

Buffer Kit for Ni-NTA, Ni-NTA Buffer

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1 kit
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CHF 218.00

Informazioni su questo articolo

NACRES:
NA.56
UNSPSC Code:
41106500

CHF 218.00


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manufacturer/tradename

Novagen®

Quality Level

storage condition

OK to freeze

shipped in

ambient

storage temp.

10-30°C

General description

The Ni-NTA Buffer Kit provides a convenient set of buffers optimized for purification of His•Tag fusion proteins on Ni-NTA His•Bind Resin. These phosphate-buffered solutions differ from the Tris-based solutions used in the His•Bind Buffer Kit. Carefully prepared 4X concentrates are included for binding, washing and elution according to recommended protocols.

Other Notes

•2 × 125 ml4X Ni-NTA Bind Buffer

•125 ml4X Ni-NTA Wash Buffer

•50 ml4X Ni-NTA Elute Buffer

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

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Questo articolo
7075169755-MGE11-0034-00
Millipore

Millipore

70899

Ni-NTA Buffer Kit

His Buffer Kit Cytiva 11-0034-00

GE11-0034-00

His Buffer Kit

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

storage temp.

10-30°C

storage temp.

-

storage temp.

2-8°C

storage temp.

-

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

-

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Cytiva 11-0034-00

shipped in

ambient

shipped in

wet ice

shipped in

-

shipped in

-


pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Numero articolo commerciale globale

SKUGTIN
70899-304055977272673

Questions

  1. Hi I want to buy this kit: 70899 Ni-NTA Buffer Kit and want to also know the buffer composition. Since I am doing native protein purification its important for me to know the composition of the buffers. Please inform where I can find it. Thanks

    1 answer
    1. Please see the composition for each of the 3 buffers included in this kit:
      Ni-NTA Bind Buffer: 50 mM NaH2PO4, pH 8.0; 300 mM NaCl; 10 mM imidazole
      Ni-NTA Wash Buffer: 50 mM NaH2PO4, pH 8.0; 300 mM NaCl; 20 mM imidazole
      Ni-NTA Elute Buffer: 50 mM NaH2PO4, pH 8.0; 300 mM NaCl; 250 mM imidazole

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