565767

α-Secretase Substrate II, Fluorogenic

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).

• Sinonimo/i: α-Secretase Substrate II, Fluorogenic, Ac-RE(EDANS)-VHHQKLVF-K(DABCYL)-R-OH
• Formula empirica (notazione di Hill): C₁₀₀H₁₄₄N₃₀O₂₀S
• Peso molecolare: 2118.47
• Codice UNSPSC: 12352200
• NACRES: NA.77

Proprietà

• Livello qualitativo: 100
• Saggio: ≥95% (HPLC)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic); protect from light
• Colore: red
• Solubilità: DMSO: 5 mg/mL
• Fluorescenza: λ_ex ~340 nm; λ_em ~490 nm
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP). Shown to be a substrate for yapsin 2, a glycosyl-phosphatidylinositol-linked yeast aspartyl protease (V_max/K_m = 0.031 min^-1). The cleavage is shown to occur at the Lys-Leu bond.

An internally quenched fluorogenic peptide substrate that contains the α-secretase cleavage site of β-amyloid precursor protein (APP). Shown to be an effective substrate for yapsin-2, a glycosyl-phosphatidylinositol-linked aspartyl protease found in yeast (V_max/K_m = 0.031 min^-1). The cleavage has been shown to occur at the Lys-Leu bond. Excitation max: ~340 nm; emission max: ~490 nm.

Azioni biochim/fisiol

Cell permeable: no

Primary Target
Substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP)

Product does not compete with ATP.

Reversible: no

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Sequenza

Ac-Arg-Glu(EDANS)-Val-His-His-Gln-Lys-Leu-Val-Phe-Lys(DABCYL)-Arg-OH

Altre note

Komano, H., et al. 1999. J. Biol. Chem. 274, 24431.
Komano, H., et al. 1998. J. Biol. Chem. 273, 31648.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable