This product is a liquid medium. The density is not reported. The 1L volume weighs approximately 1 kilogram. The total weight of the packaged 1L size is 1.1 kg. The total weight of the packaged 500 mL size is 578 g.
D6429
DMEM - high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
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동의어(들):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
보기 변경
| 팩 크기 | SKU | 재고 정보 | 가격 (VAT 별도) |
|---|---|---|---|
| 500 mL | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩52,171 | |
| 1 L | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩82,723 | |
| 24 x 500 mL | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩980,592 |
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352207
NACRES:
NA.71
제품 이름
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
General description
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s Modified Eagle′s Medium - high glucose has been used for cell culture.[1]
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이 품목 | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| form liquid | form liquid | form liquid | form liquid |
| components HEPES: no | components NaHCO3: yes | components NaHCO3: yes | components L-glutamine: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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저장 등급
12 - Non Combustible Liquids
문서
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Cell culture protocol for passaging and splitting suspension cell lines
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국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| D6429-6X1L | 04061833587577 |
| D6429-24X500ML | 04061833587553 |
| D6429-6X500ML | 04061838259363 |
| D6429-1L | 04061833587546 |
| D6429-500ML | 04061833587560 |
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How much does D6429-1L weigh? Thank you!
1 답변-
도움이 되었습니까?
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Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?
1 답변-
This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d6429#product-documentation도움이 되었습니까?
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What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?
1 답변-
This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
도움이 되었습니까?
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After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)
1 답변-
Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.
도움이 되었습니까?
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I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.
1 답변-
The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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