P4591
Pyruvate Oxidase from microorganisms
lyophilized powder, ≥1.5 U/mg
동의어(들):
Pyruvate:oxygen oxidoreductase (phosphorylating)
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크기 선택
보기 변경
| 팩 크기 | SKU | 재고 정보 | 가격 (VAT 별도) |
|---|---|---|---|
| 100 units | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩534,093 |
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-644-5
MDL number:
Specific activity:
≥1.5 U/mg
₩534,093
오늘 배송 가능세부사항
General description
Isoelectric point : 4.3
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Michaelis constant : 3.4 X 10-4M (Pyruvate)
Inhibitors : Fe++,Zn++,Cu++,Ag+,Hg++
Optimum pH : 5.7
Optimum temperature : 65oC
pH Stability : pH 5.7 - 6.5 (25oC, 20hr)
Thermal stability : below 45oC (pH 6.0, 15min)
Application
Pyruvate Oxidase from microorganisms has been used in the Amplex Red-based fluorescence assay for pyruvate. It has also been used in developing near real-time continuous detection system for pyruvate.
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.
Biochem/physiol Actions
Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbondioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Pyruvate oxidase is activated by the cofactor thiamine.
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.
Physical form
Lyophilized powder containing FAD and sugar as stabilizer
Other Notes
One unit will produce 1.0 μmole of H2O2 per min during the conversion of pyruvate and phosphate to acetylphosphate and CO2 at pH 5.7 at 37 °C.
1 of 1
이 품목 | |||
|---|---|---|---|
| specific activity ≥1.5 U/mg | specific activity ≥35 units/mg protein (biuret) | specific activity ≥50 units/mg protein | specific activity 5-15 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~260 kDa | mol wt - | mol wt 64 kDa | mol wt octomer 600 kDa by sedimentation equilibrium |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
프로토콜
Protocol for Assay Procedure for Pyruvate Oxidase Bacterial
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| P4591-100UN | 04061834374909 |