A6410
Apyrase aus Kartoffeln
ATPase ≥200 units/mg protein, lyophilized powder
Synonym(e):
Adenosin-5′-diphosphatase, Adenosin-5′-triphosphatase und Adenosin-5′-diphosphatase.
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About This Item
Empfohlene Produkte
Typ
Grade VI
Qualitätsniveau
Form
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≤15 % of base activity ADPase
Mol-Gew.
~49 kDa by gel filtration
Zusammensetzung
Protein, 30-60%
Löslichkeit
H2O: soluble 1.0 mg/mL
Fremdaktivität
Acid Phosphatase ≤2% of base activity
Lagertemp.
−20°C
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Allgemeine Beschreibung
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Anwendung
Apyrase from potatoes has been used:
- to deplete ATP
- to degrade endogenous extracellular ATP
- as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
Mindestens zwei Isoenzyme kommen in verschiedenen Varietäten von S. tuberosum vor: eines mit einem hohen ATPase/ADPase-Verhältnis (∼10) und ein weiteres mit einem niedrigen Verhältnis (∼1).
Reaktion: ATP → ADP+Pi → AMP+2Pi.
Reaktion: ATP → ADP+Pi → AMP+2Pi.
Biochem./physiol. Wirkung
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Verpackung
Sold on the basis of ATPase units.
Sonstige Hinweise
This grade has an exceptionally high ATPase/ADPase ratio.
Einheitendefinition
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
Physikalische Form
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Angaben zur Herstellung
Derived from white potato
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
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