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M7024

Sigma-Aldrich

Murashige and Skoog Shoot Multiplication Medium A

powder, suitable for plant cell culture

Synonym(e):

MSMA

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1 L
CHF 13.30
10 L
CHF 39.10

CHF 13.30


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1 L
CHF 13.30
10 L
CHF 39.10

About This Item

UNSPSC-Code:
12352207
NACRES:
NA.72

CHF 13.30


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Form

powder

Qualitätsniveau

Methode(n)

cell culture | plant: suitable

Anwendung(en)

agriculture

Versandbedingung

ambient

Lagertemp.

2-8°C

Anwendung

Murashige and Skoog Shoot Multiplication Medium A is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Alternative Formulierung

With the macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP as described by Huang and Murashige (1976) TCA Manual.

Media Formulation

Angaben zur Herstellung

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M7024 is a variant media) with macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP.

Piktogramme

Flame over circle

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Ox. Sol. 3

Lagerklassenschlüssel

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Plant tissue culture media: major constituents, their preparation and some applications.
Huang L, Murashige T.
Tissue Antigens, 3, 539-548 (1976)
R H Smith et al.
In vitro, 13(5), 329-334 (1977-05-01)
Defined in vitro conditions for callus initiation by Gossypium arboreum L. were determined, and different tissues were evaluated as explant sources, Environmental conditions tested included light versus dark, and low light versus high light. Different nutrient media as well as
Nor Azlina Hasbullah et al.
Pakistan journal of biological sciences : PJBS, 11(11), 1449-1454 (2008-09-27)
Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds
Akasaka et al.
Plant science : an international journal of experimental plant biology, 156(2), 169-175 (2000-08-11)
Bud primordia were induced from leaf segments, which were harvested from young seedlings of Spanish type peanut (Arachis hypogaea L. cv. Chico), on 0.8% agar-solidified medium containing Murashige and Skoog (MS) basal salts supplemented with B5 vitamins, 1 mg/l NAA
Regulation of lycopene formation in cell suspension culture of VFNT tomato (Lycopersicon esculentum) by CPTA, growth regulators, sucrose, and temperature
Robertson GE, Mahoney NE
Journal of Experimental Botany, 46, 667-673 (1995)

Questions

  1. In the description of M7024, it is written that the medium, in addition to macro and micronutrients, contains sucrose, IAA and Kinetin. It is also described it contains agar, but the medium did not solidify. What percentage of agar in the product?

    1 answer
    1. This product does not contain Agar. A link to the plant media formulations can be found in the DESCRIPTION section of the product page. Please see the link below:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog

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