Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf
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M9274
Murashige-Skoog-Basalmedium
suitable for plant cell culture, with sucrose and agar
Synonym(e):
MS-Basalmedium
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1 L
CHF 14.60
10 L
CHF 78.10
About This Item
Empfohlene Produkte
Qualitätsniveau
Form
powder
Methode(n)
cell culture | plant: suitable
Anwendung(en)
agriculture
Versandbedingung
ambient
Lagertemp.
2-8°C
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Anwendung
Alternative Formulierung
With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
Menge
Formulated to contain 42.4 grams of powder per liter of medium.
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Beschreibung
Preisangaben
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Ox. Sol. 3
Lagerklassenschlüssel
5.1B - Oxidizing hazardous materials
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Would an incorrect pH level affect the setting of the agar?
1 answer-
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How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
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What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
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How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
1 answer-
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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