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E5134

Ethylendiamintetraessigsäure Dinatriumsalz Dihydrat

suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

Ethylenediaminetetraacetic acid disodium salt dihydrate

Synonym(e):

Sequestren Na₂, Dinatriumethylendiamintetraacetat Dihydrat, EDTA Dinatriumsalz, EDTA-Na2, Edathamil, Edetat Dinatriumsalz Dihydrat, Sequestrene Na2

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50 g
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CHF 40.80
100 g
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250 g
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500 g
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CHF 191.00
1 kg
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5 kg
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Über diesen Artikel

Empirische Formel (Hill-System):
C10H14N2Na2O8 · 2H2O
CAS-Nummer:
Molekulargewicht:
372.24
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12352107
EC Number:
205-358-3
Beilstein/REAXYS Number:
3900609

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grade

Molecular Biology

Quality Segment

assay

99.0-101.0% (titration)

form

powder

reaction suitability

reagent type: chelator

technique(s)

electrophoresis: suitable

impurities

≤0.005% Insolubles, ≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3  M NaOH: 1.6 g/10 mL

cation traces

Fe: ≤0.005%, Pb: ≤0.002%

suitability

suitable for electrophoresis, suitable for molecular biology

foreign activity

DNase, RNase, NICKase and protease, none detected

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

General description

EDTA-Dinatriumsalz-Dihydrat wirkt als Chelator von Metallionen und bildet einen Oktaederkomplex mit divalenten Kationen. Dies ist in der molekularbiologischen Forschung nützlich, um Enzyme zu hemmen, die von der Aktivierung durch Metallionen abhängig sind. Bei diagnostischen Tests fungiert es während der Blutentnahme als Antikoagulans für hämatologische Untersuchungen

Application

EDTA-Dinatriumsalz wird in der Forschung wie folgt eingesetzt:
  • als Komponente in Pufferlösungen für Elektrophorese, Zellkultivierung, enzymatische Assays, siRNA, Durchflusszytometrie, Färbung mit Dimetylmethylblau und mehr[1]
  • als Antikoagulans bei der Vollblutentnahme
  • als Stabilisatorlösung zum Einfrieren von Zellkulturproben[2]
  • in Kombination mit PBS und Trypsin zur Trypsinisierung von Zellen[3]
  • für die Phytoextraktion von Schwermetallen in verschmutzten Böden
Chelatbildner mit divalenten Kationen. Blockiert Enzyme wie Metallproteasen, die für ihre Aktivität divalente Kationen benötigen.

Features and Benefits

  • Molekularbiologische Qualität, geeignet für viele molekularbiologische Anwendungen
  • Frei von Endonukleasen, DNase, RNase, Nickase und Protease

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Dieser Artikel
ED2SS27285E4884
grade

Molecular Biology

grade

reagent grade

grade

-

grade

ACS reagent

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

99.0-101.0% (titration)

assay

98.5-101.5% (titration)

assay

99.0-101.0%

assay

99.0-101.0%

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

impurities

≤0.005% Insolubles, ≤0.1% Nitrilotriacetic acid (NTA)

impurities

-

impurities

residual solvents, complies, ≤0.001% cyanide (CN), ≤0.001% heavy metals (as Pb), ≤0.1% nitrilotriacetic acid (HPLC) (HPLC)

impurities

≤0.005% Insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

form

powder

form

powder

form

crystals

form

solid


Still not finding the right product?


pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Lagerklasse

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Analysenzertifikate (COA)

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Questions

1–7 of 7 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Product E5134 has a re-test date of Aug. 2023. Has the re-test been completed?

    1 answer
    1. The specific lot number is required to determine the retest status. You may obtain this lot status by contacting our Technical Service team. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information, including the specific lot number, so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

  4. What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?

    1 answer
    1. The optimal concentration is 1.5 mg per mL of blood.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. Is Product E5134, EDTA disodium salt, stable in solution?

    1 answer
    1. Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.

      Helpful?

  7. Can solutions of Product E5134, EDTA disodium salt, be autoclaved?

    1 answer
    1. Yes.

      Helpful?

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