C8571

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

• Synonym(e): Cathepsin B₁
• CAS-Nummer: 9047-22-7
• EC-Nummer: 3.4.22.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130748
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human liver
• Qualitätsniveau: 200
• Form: buffered aqueous solution
• Spezifische Aktivität: ≥1,500 units/mg protein (E1%/280)
• UniProt-Hinterlegungsnummer: P07858
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... CTSB(1508)

Beschreibung

Allgemeine Beschreibung

Cathepsin B gene is mapped to human chromosome 8p23.1.[1] It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence.[2] It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.[3]

Anwendung

Cathepsin B from human liver has been used:

• as a positive control in the screening Cathepsin B inhibitor assay[4]
• in wall extension (expansin activity) assay[5]
• in microinjection human foreskin fibroblasts to test its effect on apoptosis induction[6]

Biochem./physiol. Wirkung

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC).[1] It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.[7]

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Verpackung

Package size based on protein content

Einheitendefinition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physikalische Form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable