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Merck

F6257

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Sheep Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

sheep

Konjugat

FITC conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Methode(n)

direct immunofluorescence: 1:32

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for all mouse IgG subclasses. Affinity isolated antigen specific antibody is obtained from sheep anti-mouse IgG antiserum by immunospecific purification. The antibody preparation is then conjugated to Sigma Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified mouse IgG

Anwendung

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a working antibody dilution of 1:32. For immunofluorescence of human ovarian adenocarcinoma cells stained with anti-integrin primary antibodies a working dilution of 1:50 was used. Immunolocalization of human spermatids was done using antibody dilution of 1:100. This antibody was also used for immunofluorescent tagging of leaf cells of Arabidopsis.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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