F2012

Anti-Maus-IgG (Gesamtmolekül) – FITC in Ziege hergestellte Antikörper

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate
• MDL-Nummer: MFCD00162642
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: goat
• Konjugat: FITC conjugate
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Methode(n): direct immunofluorescence: 1:64 (minimum)
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

The product binds all mouse Igs.

Anwendung

Anti-Mouse IgG (whole molecule)−FITC antibody has been used:

• in immunofluorescence
• in immunohistochemistry
• in immunofluorescence staining
• in immunofluorescence microscopy
• in confocal imaging

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines.

Biochem./physiol. Wirkung

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Sonstige Hinweise

Antibody adsorbed with human serum proteins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Angaben zur Herstellung

Adsorbed to reduce background staining with human samples

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable