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A2289

Albumin, Tetramethylrhodamine isothiocyanate bovine

Name: Albumin, Tetramethylrhodamine isothiocyanate bovine
Brand: SIGMA
Price: 382 CHF

lyophilized powder

Synonym(e):

TRITC-albumin

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Packungsgröße	SKU	Verfügbarkeit	Preis
10 mg		Warenkorb auf Verfügbarkeit prüfen	CHF 382.00
50 mg		Warenkorb auf Verfügbarkeit prüfen	CHF 1’540.00

Über diesen Artikel

CAS-Nummer:

9048-46-8

UNSPSC Code:

12352202

NACRES:

NA.25

MDL number:

MFCD00130416

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥90% (Lowry)

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Impurities:

salt, free

Concentration:

≥90% protein (Lowry)

CHF 382.00

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Eigenschaften

biological source

bovine

Quality Segment

200

assay

≥90% (Lowry)

form

lyophilized powder

concentration

≥90% protein (Lowry)

extent of labeling

≥0.5 mol TRITC per mol albumin

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Beschreibung

General description

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Application

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

Albumin, Tetramethylrhodamine isothiocyanate has been used:

in photoinduced release assay of polyethylene beads
for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
in fluorescent correlation spectroscopy

Preparation Note

Prepared from crystallized and lyophilized albumin (A4378).

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Dieser Artikel	N8159	A3264	A8426
Sigma-Aldrich A2289 Albumin, Tetramethylrhodamine isothiocyanate bovine	Sigma-Aldrich N8159 Albumin, Nitrated from bovine serum	Sigma-Aldrich A3264 Albumin from sheep serum	Sigma-Aldrich A8426 Albumin, glycated bovine
assay ≥90% (Lowry)	assay -	assay ≥97% (agarose gel electrophoresis)	assay ≥90% protein basis (biuret)
technique(s) cell culture \| mammalian: suitable	technique(s) immunoblotting: suitable	technique(s) hybridization: suitable, microbiological culture: suitable, protein quantification: suitable	technique(s) cell culture \| mammalian: suitable
biological source bovine	biological source bovine	biological source sheep	biological source bovine
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
Gene Information bovine ... ALB(280717)	Gene Information bovine ... ALB(280717)	Gene Information sheep ... ALB(443393)	Gene Information bovine ... ALB(280717)