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63689

2-Mercaptoethanol

BioUltra, Molecular Biology, ≥99.0% (GC)

Synonym(e):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylenglycol

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PackungsgrößeSKUVerfügbarkeitPreis
25 mL
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CHF 44.80
100 mL
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CHF 124.00

Über diesen Artikel

Lineare Formel:
HSCH2CH2OH
CAS-Nummer:
Molekulargewicht:
78.13
UNSPSC Code:
12161504
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:

CHF 44.80


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grade

Molecular Biology

Quality Segment

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.0% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

RNA extraction: suitable, protein extraction: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.500 (lit.), n20/D 1.501

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.114 g/mL at 25 °C (lit.)

SMILES string

OCCS

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤1 mg/kg, Ni: ≤0.05 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 1.5, λ: 280 nm Amax: 0.3

storage temp.

2-8°C

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

Application

2-Mercaptoethanol has been used
  • in the protein extraction buffer prepared for leaf samples.[1]
  • as a supplement in cell culture media.
  • in the procedure of RNA extraction.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds[2]

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Dieser Artikel
M314897622PHR9274
technique(s)

RNA extraction: suitable, protein extraction: suitable

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

HPLC: suitable

technique(s)

-

grade

Molecular Biology

grade

Molecular Biology

grade

derivatization grade (HPLC)

grade

certified reference material, TraceCERT®

assay

≥99.0% (GC)

assay

99% (GC/titration)

assay

≥99.0% (GC)

assay

-

product line

BioUltra

product line

BioReagent

product line

-

product line

TraceCERT®

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

300

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C


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Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Lagerklasse

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Protokolle

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Überblick über die Chemie von Polyacrylamidgelen und detaillierte Anweisungen für den Handguss von Polyacrylamidgelen mit den mPAGE® TurboMix Bis-Tris-Gelgießkits.

Vue d'ensemble des propriétés chimiques des gels de polyacrylamide et détail des consignes à suivre pour les couler à l'aide des kits de coulage de gels Bis-Tri mPAGE® TurboMix.

Alle Protokolle anzeigen

Artikel

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.


Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.
Benitez JJ et al.
Lab on a chip, 12, 4848-4848 (2012)
The interplay between protein L-isoaspartyl methyltransferase activity and insulin-like signaling to extend lifespan in Caenorhabditis elegans.
Khare S et al.
PLoS ONE, 6, e20850-e20850 (2011)
Adam Healey et al.
Plant methods, 10, 21-21 (2014-07-24)
Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA extraction difficult. While many DNA extraction



Global Trade Item Number

SKUGTIN
63689-1ML-KC-F04061838084859
63689-100ML-F04061838357557
63689-25ML-F04061832723167

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Is this product suitable for cell culture?

    1 answer
    1. This product has not been tested for use in cell culture and is listed as a molecular biology grade material. See product M3148 at the link below to review the cell culture tested product option:
      https://www.sigmaaldrich.com/product/sigma/m3148

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. How do I know when 2-Mercaptoethanol expires

    1 answer
    1. This material is quite stable when stored and handled properly. One visual indication of degradation would be a color change to a darker yellow to brown. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

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