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F7258

Fast Green FCF

certified by the BSC

Synonym(e):

Lebensmittelgrün 3

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Ihnen/SKUVerfügbarkeitPreis
25 g
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CHF 249.00

Über diesen Artikel

Lineare Formel:
C37H34N2O10S3Na2
CAS-Nummer:
Molekulargewicht:
808.85
EC Number:
219-091-5
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5718212
Colour Index Number:
42053

CHF 249.00


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agency

certified by the BSC

Quality Segment

form

powder

composition

dye content, ≥90%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Zertifiziert für die Safranin-Echtgrün FCF-Färbung von Pflanzenzellen; als Ersatz für Anilinblau in der Einstufen-Gomori-Trichrom-Färbung.
Fast Green FCF has been used:
  • along with safranin for the staining of nodule sections
  • in the collagen deposit staining procedure[1]
  • for the staining of protein and starch
  • as a dye to detect and inject DNA solution in chicken embryo

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Dieser Artikel
F7252B4014B6756
solubility

H2O: 1 mg/mL

solubility

H2O: 1 mg/mL, clear

solubility

H2O: 1 mg/mL, blue

solubility

H2O: 1 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

agency

certified by the BSC

agency

-

agency

certified by the BSC

agency

-

mp

290 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

mp

210 °C (dec.) (lit.)

mp

210 °C (dec.) (lit.)


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pictograms

Health hazardExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Lagerklasse

11 - Combustible Solids

wgk

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup

ppe

Eyeshields, Gloves



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Questions

1–2 of 2 Questions  
  1. What is the proper method to prepare Fast Green FCF for use as a counterstain?

    1 answer
    1. Solutions of Fast Green FCF are used much like solutions of Light Green SF Yellowish. The advantage of using Fast Green FCF is generally that the stain is more intense. The working solution is typically prepared fresh each day or used for a short period of time.

      Helpful?

  2. Looking for a green dye to add to electrolyzed water. Something biodegradable that will disappear fast on skin.

    1 answer
    1. It is advised to source a FD&C (Food, Drug & Cosmetic) grade dye for use on human skin. Assuming there is suitable electrolyzed water, green food coloring should suffice for the intended purpose. The duration for which the dye remains on the skin would depend on factors such as the pH of the water used and the specific characteristics of the dye, which the customer would need to determine.

      Helpful?

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